Pengaruh Naungan terhadap Kandungan Total Flavonoid dan Kapasitas Antioksidan Ekstrak Daun Miana (Coleus scutellarioides (L.) Benth.)

Abstract

Tanaman miana (Coleus scutellarioides (L.) Benth) mengandung senyawa flavonoid yang berpotensi sebagai antioksidan alami. Penelitian ini bertujuan mengukur kandungan flavonoid dan kapasitas antioksidan daun miana pada tingkat naungan 0%, 25%, 50%, dan 75%. Kadar flavonoid dianalisis dengan metode kalorimetri aluminium klorida (AlCl3), sementara kapasitas antioksidan dengan metode 2,2-azinobis (3-etilbenzotiazolin-6-asam sulfonat) (ABTS) dan cupric ion reducing antioxidant capacity (CUPRAC). Hasil menunjukkan naungan 25% memiliki kadar flavonoid tertinggi sebesar 1,116 ± 0,012 mg QE/g DW, sementara kapasitas antioksidan tertinggi diperoleh pada naungan 0% sebesar 1,979 ± 0,006 µM TE/g DW (ABTS) dan 184,427 ± 2,894 µM TE/g DW (CUPRAC). Nilai kapasitas antioksidan lebih tinggi pada metode CUPRAC menunjukkan bahwa metode ini lebih representatif dalam mencerminkan kapasitas antioksidan daun miana. Korelasi positif terjadi antara kadar flavonoid dan kapasitas antioksidan ABTS maupun CUPRAC pada perlakuan naungan 25% dan 50%, sementara korelasi bersifat negatif pada perlakuan naungan 0% dan 75%. Hal ini menunjukkan intensitas cahaya memengaruhi kandungan senyawa bioaktif daun miana.

The miana plant (Coleus scutellarioides (L.) Benth) contains flavonoid compounds that have potential as natural antioxidants. This study aimed to measure the flavonoid content and antioxidant capacity of miana leaves under shading levels of 0%, 25%, 50%, and 75%. Flavonoid content was analyzed using the aluminum chloride calorimetry (AlCl3) method, while antioxidant capacity was determined using the 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and cupric ion reducing antioxidant capacity (CUPRAC) methods. The results showed that 25% shading had the highest flavonoid content of 1.116 ± 0.012 mg QE/g DW, while the highest antioxidant capacity was obtained at 0% shading at 1.979 ± 0.006 µM TE/g DW (ABTS) and 184.427 ± 2.894 µM TE/g DW (CUPRAC). The higher antioxidant capacity values in the CUPRAC method indicate that this method is more representative in reflecting the antioxidant capacity of miana leaves. A positive correlation was observed between flavonoid content and both ABTS and CUPRAC antioxidant capacities under 25% and 50% shading treatments, while a negative correlation was observed under 0% and 75% shading treatments. This indicates that light intensity affects the content of bioactive compounds in miana leaves.