Evaluasi Kualitas Sinbiotik Air Kelapa Fermentasi Saccharomyces cerevisiae dan Lactobacillus plantarum sebagai Aditif Pakan untuk Ayam Broiler

Abstract

Pelarangan penggunaan Antibiotic Growth Promoter (AGP) mendorong pengembangan sinbiotik sebagai aditif pakan alami bagi ayam broiler. Penelitian ini bertujuan mengevaluasi kualitas sinbiotik air kelapa fermentasi (AKF) berdasarkan viabilitas mikroba, aktivitas antimikroba, dan daya simpan. Air kelapa tua difermentasi menggunakan Lactobacillus plantarum (AKF-Lp), Saccharomyces cerevisiae (AKF-Sc), dan kombinasinya (AKF-Mix). Viabilitas mikroba dianalisis menggunakan metode Total Plate Count (TPC), aktivitas antimikroba diuji terhadap Escherichia coli dan Salmonella sp., sedangkan daya simpan dievaluasi berdasarkan perubahan pH selama lima minggu penyimpanan. Pada produksi kedua, AKF-Sc dan AKF-Mix menghasilkan nilai TPC tertinggi, masing-masing 6,66 dan 6,70 log CFU/ml, serta memenuhi standar minimum probiotik (=6 log CFU/ml). AKF-Sc menghasilkan diameter zona hambat tertinggi terhadap E. coli (10,12 mm) dan Salmonella sp. (10,17 mm). Penyimpanan pada suhu refrigerator mempertahankan pH lebih baik (4,27) dibandingkan suhu ruang (3,63). Berdasarkan ketiga parameter tersebut, AKF berbasis S. cerevisiae berpotensi dikembangkan sebagai aditif pakan alami pada ayam broiler.

The ban on Antibiotic Growth Promoters (AGP) has driven the development of synbiotics as natural feed additives for broiler chickens. This study aimed to evaluate the quality of fermented coconut water (FCW) synbiotics based on microbial viability, antimicrobial activity, and shelf life. Mature coconut water was fermented using Lactobacillus plantarum (FCW-Lp), Saccharomyces cerevisiae (FCW-Sc), and a combination of both (FCW-Mix). Microbial viability was analyzed using the Total Plate Count (TPC) method, antimicrobial activity was tested against Escherichia coli and Salmonella sp., and shelf life was evaluated based on pH changes over five weeks of storage. In the second production run, FCW-Sc and FCW-Mix yielded the highest TPC values 6,66 and 6,70 log CFU/ml, respectively thereby meeting the minimum probiotic standard (=6 log CFU/ml). FCW-Sc produced the largest inhibition zone diameters against E. coli (10,12 mm) and Salmonella sp. (10,17 mm). Refrigerated storage maintained the pH better (4,27) compared to room temperature storage (3,63). Based on these three parameters, S. cerevisiae based FCW shows potential for development as a natural feed additive for broiler chickens.