Karakterisai dan Morfologi Halococcus salsus dan Haloferax volcanii yang Ditumbuhkan pada Media Hidrolisat Protein Ikan

Abstract

Arkea halofilik ekstrem seperti Halococcus salsus dan Haloferax volcanii memiliki potensi bioteknologi yang luas, namun umumnya memerlukan media pertumbuhan dengan komponen kompleks dan relatif mahal. Penelitian ini bertujuan mengkarakterisasi pertumbuhan dan morfologi kedua arkea tersebut pada media hidrolisat protein ikan (HPI) sebagai sumber alternatif. Media HPI diformulasikan dengan konsentrasi garam tinggi sesuai kebutuhan arkea halofilik. Karakterisasi dilakukan melalui pengamatan kurva pertumbuhan, morfologi sel menggunakan Scanning Electron Microscopy (SEM), pewarnaan Gram, serta uji respon terhadap cekaman oksidatif (H2O2) dan ion logam. Hasil penelitian menunjukkan media HPI mampu mendukung pertumbuhan kedua arkea halofilik. Analisis SEM menunjukkan Halococcus salsus berbentuk bulat, sedangkan Haloferax volcanii berbentuk pleomorfik, pewarnaan Gram menunjukkan reaksi Gram-negatif. Uji respon oksidatif dan ion logam menunjukkan adanya variasi tingkat toleransi yang rendah antara kedua spesies. Secara keseluruhan, HPI berpotensi sebagai media alternatif untuk mendukung pertumbuhan dan karakterisasi arkea halofilik.

Extreme halophilic archaea such as Halococcus salsus and Haloferax volcanii have broad biotechnological potential, but they generally require growth media with complex and relatively expensive components. This study aimed to characterize the growth and morphology of Halococcus salsus and Haloferax volcanii cultivated on fish protein hydrolysate (FPH) medium as an alternative source. The FPH medium was formulated with a high salt concentration according to the requirements of halophilic archaea. Characterization was carried out by observing growth curves, cell morphology using Scanning Electron Microscopy (SEM), Gram staining, and tests of responses to oxidative stress (H2O2) and metal ions. The results showed that FPH medium was able to support the growth of both halophilic archaea. SEM analysis revealed that Halococcus salsus has a spherical shape, while Haloferax volcanii exhibits a pleomorphic shape, while Gram staining showed a Gram-negative reaction. Oxidative stress and metal ion response tests showed slight variations in tolerance levels between the two species. Overall, fish protein hydrolysate has the potential to be used as an alternative medium to support the growth and characterization of halophilic archaea.