Kapasitas Antioksidan dan Inhibisi Lipase Pankreas Kombinasi Daun Kumis Kucing (Orthosiphon Aristatus) dan Daun Camellia sinensis Camellia sinensis

Abstract

Kumis kucing (Orthosiphon aristatus) dan Camellia sinensis merupakan tanaman yang dilaporkan memiliki potensi sebagai agen antiobesitas. Penelitian ini bertujuan menentukan efek kombinasi berdasarkan kadar fenolik total, kadar flavonoid total, kapasitas antioksidan, dan inhibisi lipase pankreas dari kombinasi ekstrak Orthosiphon aristatus dan Camellia sinensis. Metode ekstraksi menggunakan sonikasi dengan etanol 70% kemudian sampel dikombinasikan menjadi tujuh jenis kombinasi. Setiap kombinasi diuji kandungan fitokimia, antioksidan, dan inhibisi lipase pankreas. Hasil penelitian menunjukkan kadar fenolik total, kadar flavonoid total, kapasitas antioksidan DPPH, dan kapasitas antioksidan FRAP memiliki efek sinergis berdasarkan peningkatan nilai dibandingkan nilai teoretis kombinasi ekstrak tunggal. Sementara itu, inhibisi lipase pankreas menunjukkan efek antagonis karena menunjukkan penurunan nilai dibandingkan nilai teoretis kombinasi ekstrak tunggal.

Cat's Whiskers (Orthosiphon aristatus) and Camellia sinensis are plants reported to have potential as anti-obesity agents. This study aims to determine the combination effect based on total phenolic content, total flavonoid content, antioxidant capacity, and pancreatic lipase inhibition of the combined extracts of Orthosiphon aristatus and Camellia sinensis. The extraction method used sonication with 70% ethanol, and the samples were then formulated into seven different combinations. Each combination was evaluated for its phytochemical content, antioxidant capacity, and pancreatic lipase inhibition. The results showed that the total phenolic content, total flavonoid content, DPPH antioxidant capacity, and FRAP antioxidant capacity exhibited a synergistic effect, as indicated by an increase in values compared to the theoretical values of the single extract combinations. Meanwhile, pancreatic lipase inhibition demonstrated an antagonistic effect, as evidenced by a decrease in values compared to the theoretical values of the single extract combinations.