Kapasitas Antioksidan dan Inhibisi Lipase Pankreas Hasil Fraksinasi Serai Wangi (Cymbopogon Nardus) In vitro

Abstract

Obesitas memiliki faktor risiko yang tinggi dan dapat ditangani dengan menghambat enzim lipase pankreas. Obesitas memicu stres oksidatif yang dapat dicegah dengan antioksidan oleh senyawa fenolik dan flavonoid. Serai wangi (Cymbopogon nardus) belum diketahui potensi fraksinasinya sebagai inhibitor lipase pankreas secara in vitro. Penelitian ini bertujuan mencari fraksi terbaik pada kadar fenolik dan flavonoid total serta kapasitas antioksidan dan inhibisi lipase pankreas. Serai wangi diekstraksi dengan etanol 70% lalu dilanjutkan fraksinasi bertingkat dengan pelarut n-heksan, kloroform, etil asetat, dan air lalu dihitung rendemennya. Selanjutnya diuji kadar fenolik dan flavonoid total serta kapasitas antioksidan dari metode DPPH dan FRAP, lalu diuji daya inhibisi terhadap lipase pankreas. Hasil penelitian menunjukkan bahwa kadar fenolik total tertinggi terdapat pada fraksi air, kadar flavonoid total pada fraksi kloroform, kapasitas antioksidan metode DPPH dan FRAP pada fraksi etil asetat. Penghambatan lipase pankreas baik ekstrak kasar, fraksi, ataupun orlistat tidak berbeda nyata. Perbedaan kepolaran berpengaruh pada tiap uji karena afinitas yang berbeda terhadap sifat kepolaran

Obesity has a high risk factor and can be treated by inhibiting the pancreatic lipase enzyme. Obesity triggers oxidative stress which can be prevented by antioxidants by phenolic compounds and flavonoids. Citronella (Cymbopogon nardus) has no known fractionation potential as an inhibitor of pancreatic lipase in vitro. This research aimed to find the best fraction for total phenolic and flavonoid levels as well as antioxidant capacity and pancreatic lipase inhibition. Citronella was extracted with 70% ethanol then continued with multilevel fractionation with the solvent n-hexane, chloroform, ethyl acetate and water and then the yield was calculated. Next, total phenolic and flavonoid levels were tested as well as antioxidant capacity from the DPPH and FRAP methods, then the inhibitory power against pancreatic lipase was tested. The results showed that the highest total phenolic content was in the water fraction, total flavonoid content in the chloroform fraction, antioxidant capacity of the DPPH and FRAP methods in the ethyl acetate fraction. Inhibition of pancreatic lipase, whether crude extract, fraction or orlistat, was not significantly different. Differences in polarity affect each test because of different affinities for polar properties.