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      Preferensi Thraustochytrid Terhadap Beberapa Jenis Umpan Serbuk Sari

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      Date
      2023
      Author
      Nabilah, Salma
      Suwanto, Antonius
      Sulistyaningsih, Yohana Caecilia
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      Abstract
      Omega-3, asam lemak esensial dengan sifat terapeutik untuk berbagai penyakit, kini semakin sulit diperoleh dari pasokan ikan laut liar yang terbatas dan mahal. Thraustochytrid yang merupakan mikroorganisme laut, diketahui mampu menghasilkan asam lemak essensial ini. Thraustochytrid umumnya diisolasi dengan menggunakan serbuk sari dari tumbuhan pinus (Pinus sp.) sebagai umpan (pollen baiting). Belum ada penelitian terkait penggunaan serbuk sari dari tumbuhan lain yang mungkin memberikan hasil lebih baik. Penelitian ini bertujuan membandingkan sifat serbuk sari dari beberapa spesies tumbuhan dalam hal efektivitasnya sebagai umpan thraustochytrid. Serbuk sari yang digunakan berasal dari tumbuhan pinus (Pinus merkusii), kelapa (Cocos nucifera), jagung (Zea mays), sawit (Elaeis guineensis), dan kembang sepatu (Hibiscus rosa sinensis). Thraustochytrid yang berhasil terumpan oleh serbuk sari kemudian ditumbuhkan di media agar di cawan dan populasinya dihitung. Hasil pengamatan menunjukkan bahwa thraustochytrid dapat dipancing dengan serbuk sari yang mengapung di air seperti serbuk sari dari tumbuhan pinus, kelapa, dan sawit. Populasi koloni thraustochytrid tertinggi yang berhasil diisolasi ditemukan pada sampel air laut yang diumpan menggunakan serbuk sari dari tumbuhan kelapa dengan nilai 3.37 x 104 CFU/mL. Serbuk sari dari tumbuhan kelapa dapat dijadikan alternatif dalam metode pollen baiting untuk mengisolasi thraustochytrid.
       
      Omega-3, an essential fatty acid with therapeutic properties for various diseases, is becoming increasingly difficult to obtain from the limited and expensive supply of wild marine fish. Thraustochytrids, which are marine microorganisms, are known to produce these essential fatty acids. Thraustochytrids are generally isolated by using pollen from pine plants (Pinus sp.) as bait (pollen baiting). There has been no research related to the use of pollen from other plants that might provide better results. This study aims to compare the pollen properties of several plant species in terms of their effectiveness as thraustochytrid bait. The pollen used came from pine (Pinus merkusii), coconut (Cocos nucifera), corn (Zea mays), palm (Elaeis guineensis), and hibiscus (Hibiscus rosa sinensis). Thraustochytrids that were successfully baited by pollen were then grown on agar media in cups, and their populations were counted. Observations showed that thraustochytrids can be baited with pollen floating in water, such as pollen from pine, coconut, and palm plants. The highest population of thraustochytrid colonies successfully isolated was found in seawater samples baited with coconut pollen, with a value of 3.37 x 104 CFU/mL. Coconut pollen can be used as an alternative to the pollen baiting method to isolate thraustochytrids.
       
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      http://repository.ipb.ac.id/handle/123456789/125768
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      • UT - Biology [2400]

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      Copyright © 2020 Library of IPB University
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      Indonesia DSpace Group 
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