| dc.contributor.advisor | Ambarsari, Laksmi | |
| dc.contributor.advisor | Sugita, Purwantiningsih | |
| dc.contributor.author | Nur, Najma Aulia | |
| dc.date.accessioned | 2023-09-01T07:17:54Z | |
| dc.date.available | 2023-09-01T07:17:54Z | |
| dc.date.issued | 2023 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/124492 | |
| dc.description.abstract | Demam Berdarah Dengue (DBD) merupakan penyakit menular yang disebabkan oleh infeksi virus dengue (DENV). NS5 RNA-dependent RNA polymerase (RdRp) merupakan salah satu protein non-struktural yang berperan penting dalam replikasi virus dengue. Balapiravir merupakan senyawa yang telah diuji secara klinis dapat menghambat aktivitas polimerase pada virus, tetapi senyawa ini memiliki permeasi yang buruk. Biflavonoid genus Araucaria telah diteliti memiliki potensi sebagai antivirus. Penelitian ini bertujuan mengidentifikan senyawa biflavonoid genus Araucaria yang berpotensi menghambat DENV NS5 RdRp secara in silico melalui penambatan molekuler. Penambatan molekuler dilakukan menggunakan aplikasi YASARA Structure. Hasil penelitian menunjukkan 4 senyawa biflavonoid genus Araucaria yang berpotensi sebagai inhibitor DENV NS5 RdRp berdasarkan energi bebas ikatan (∆G), konstanta disosiasi (Kd), dan daerah interaksi. Senyawa 7’’-O-methyl-robustaflavone merupakan senyawa terbaik yang memiliki potensi sebagai inhibitor DENV NS5 RdRp dengan nilai ∆G dan Kd sebesar -10.3540 kkal/mol dan 2,57 x 104 pM. | id |
| dc.description.abstract | Dengue Hemorrhagic Fever (DHF) is an infectious disease caused by infection with the dengue virus (DENV). NS5 RNA-dependent RNA polymerase (RdRp) is a non-structural protein that plays an important role in dengue virus replication. Balapiravir is a compound that has been clinically tested to inhibit polymerase activity in viruses, but this compound has poor permeation. Biflavonoids of the genus Araucaria have been studied for their potential as antivirals. This study aimed to identify biflavonoid compounds of the genus Araucaria that have the potential to inhibit DENV NS5 RdRp in silico through molecular docking. Molecular docking was performed using the YASARA Structure application. The results showed that 4 biflavonoid compounds of the Araucaria genus had potential as DENV NS5 RdRp inhibitors based on bond free energy (∆G), dissociation constant (Kd), and interaction site. Compound 7''-O-methyl-robustaflavone is the best compound that has potential as a DENV NS5 RdRp inhibitor with ∆G and Kd values of -10.3540 kcal/mol and 2,57 x 104 pM. | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Simulasi Penambatan Molekuler Biflavonoid Genus Araucaria sebagai Inhibitor DENV NS5 RNA-Dependent RNA Polymerase | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | Dengue | id |
| dc.subject.keyword | NS5 RdRp | id |
| dc.subject.keyword | Biflavonoid | id |
| dc.subject.keyword | Araucaria | id |
| dc.subject.keyword | Molecular Docking | id |
