Isolasi dan Karakterisasi Gen SiCCR1 terkait Biosintesis Lignin pada Hotong (Setaria italica [L.] Beauv) untuk Sifat Toleransi terhadap Cekaman Abiotik
Date
2023Author
Listari, Bella Wanti
Ardie, Sintho Wahyuning
Suwarno, Willy Bayuardi
Metadata
Show full item recordAbstract
Hotong (Setaria italica [L.] Beauv) merupakan tanaman penghasil
karbohidrat bernutrisi baik dengan toleransi terhadap cekaman kekeringan dan
salinitas. Kandungan lignin di akar dan batang telah dilaporkan sebagai salah satu
mekanisme penting toleransi tanaman terhadap cekaman kekeringan dan salinitas,
dimana lignin berperan dalam ketegaran mekanik tanaman serta transportasi air dan
solut melalui jaringan pembuluh. Cinnamoyl-CoA reductase (CCR) merupakan
salah satu enzim kunci dalam lintasan biosintesis lignin. Penelitian ini bertujuan
mengisolasi dan mengkarakterisasi gen penyandi CCR, SiCCR1, dari empat
genotipe hotong dengan toleransi berbeda terhadap cekaman kekeringan dan
salinitas. Gen SiCCR1 berhasil diisolasi dari DNA genom satu genotipe hotong
toleran (ICERI-6) dan tiga genotipe hotong peka (NTB-1, Mauliru-2, dan Buru)
dengan panjang basa nukleotida 2.293-2.296 pb. Pensejajaran dengan gen SiCCR1
referensi dari hotong kultivar IC-403579 menunjukkan terdapat synonymous SNP
di intron 1 (basa 40, 41, 42) dan intron 4 (basa 1.398), dan satu non-synonymous
SNP di ekson 6 (basa 2.336). SNP yang teridentifikasi pada gen SiCCR1 dari
keempat genotipe hotong dalam penelitian ini belum dapat membedakan dengan
konsisten genotipe toleran dan peka terhadap cekaman kekeringan dan salinitas. Foxtail millet (Setaria italica [L.] Beauv) is one of the nutritious
carbohydrate-producing crops with comparable tolerance to drought and salinity
stresses. Lignin content in the roots and stems has been reported as one important
mechanism in the tolerance to drought and salinity stresses, where lignin plays a
role in the mechanical robustness of plants and facilitates the transportation of
water and solutes through vascular tissue. Cinnamoyl-CoA reductase (CCR) is one
of the key enzymes in the lignin biosynthesis pathway. This study aimed to isolate
and characterize the CCR encoding gene, SiCCR1, from four foxtail millet
genotypes differing in their drought- and salinity tolerance. SiCCR1 genes were
successfully isolated from the genomic DNA of one tolerant genotype (ICERI-6)
and three sensitive genotypes (NTB-1, Mauliru-2, and Buru) with the nucleotide
length ranging from 2.293-2.296 bp. Alignment with the reference sequence of
SiCCR1 from cv. IC-403579 (tolerant) showed synonymous SNPs in intron 1 (nt.
40, 41, and 42) and intron 4 (nt. 1.398), and a non-synonymous SNP in exon 6 (nt.
2.336). None of the identified SNPs were consistently associated with the tolerance
level of the foxtail millet genotypes.