| dc.contributor.advisor | Tjahjoleksono, Aris | |
| dc.contributor.advisor | Miftahudin | |
| dc.contributor.author | Aziz, Abdul | |
| dc.date.accessioned | 2023-08-07T04:37:19Z | |
| dc.date.available | 2023-08-07T04:37:19Z | |
| dc.date.issued | 2023-08-07 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/123150 | |
| dc.description.abstract | Kelarutan aluminium (Al) yang tinggi dan pH rendah menjadi faktor
pembatas pertumbuhan dan produksi tanaman kentang. IPB CP3 ialah kultivar
kentang industri yang cocok untuk pembuatan keripik kentang dan french fries. Gen
MmCu/Zn-SOD telah diintroduksi ke dalam kultivar IPB CP3 sehingga
menghasilkan kentang transgenik yang tahan cekaman abiotik. Penelitian ini
bertujuan menganalisis stabilitas transgen MmCu/Zn-SOD dan ketahanan kultivar
IPB CP3 transgenik terhadap cekaman Al dan pH rendah secara in vitro. Percobaan
dilakukan menggunakan rancangan acak lengkap (RAL), dua faktor perlakuan, dan
empat ulangan. Faktor pertama yaitu genotipe kentang yang terdiri atas IPB CP3
non-transgenik dan empat klon transgenik (P3SOD3, P3SOD7, P3SOD10 dan
P3SOD13). Faktor kedua yaitu cekaman Al dan pH rendah pada media tanam yang
terdiri atas (1) pH 4+Al 2 mM, (2) pH 4 tanpa Al, dan (3) pH 5,8 tanpa Al (kontrol).
Transgen MmCu/Zn-SOD di dalam genom tanaman IPB CP3 transgenik tetap stabil.
Tingkat ketahanan klon transgenik terhadap Al dan pH rendah bervariasi. Klon
P3SOD3 dan P3SOD10 mempunyai tingkat ketahanan paling tinggi terhadap
cekaman Al dan pH rendah. | id |
| dc.description.abstract | The high solubility of aluminum (Al) and low pH is a limiting-factors for
growth and can reduce plant productivity. IPB CP3 is an industrial potato suitable
for the manufacture of potato chips and french fries. IPB CP3 cultivar potato was
introduced with the MmCu/Zn-SOD gene resulting in abiotic stress resistant
transgenic potatoes. This study aims to analyze the stability of the MmCu/Zn-SOD
transgene in transgenic IPB CP3 potato cultivars and their resistance to Al stress
and low pH in vitro. The experiment was conducted using a completely randomized
design (CRD), two treatment factors, and four replications. The first factor was the
potato genotypes consisting of non-transgenic IPB CP3 and four transgenic clones
(P3SOD3, P3SOD7, P3SOD10, and P3SOD13). The second factor was the Al and
low pH stresses on planting medium which consisted of (1) pH 4+Al 2 mM, (2) pH
4 without Al, and (3) pH 5.8 without Al (control). The MmCu/Zn-SOD transgene
in the genome of the transgenic IPB CP3 plants remained stable. The resistance
levels of transgenic clones to Al and low pH varied. The P3SOD3 and P3SOD10
clones had the highest level of resistance to Al and low pH stresses. | id |
| dc.description.sponsorship | Kemendikbud Ristek dan Kedaireka | id |
| dc.language.iso | id | id |
| dc.publisher | IPB University | id |
| dc.title | Analisis Ketahanan Kentang Kultivar IPB CP3 Transgenik yang Membawa Gen MmCu/Zn-SOD terhadap Cekaman Aluminium dan pH Rendah | id |
| dc.title.alternative | Resistance Analysis of Transgenic Potato Cultivar IPB CP3 Containing the MmCu/Zn-SOD Gene against Aluminum Stress and Low pH | id |
| dc.type | Undergraduate Thesis | id |
| dc.subject.keyword | french fries | id |
| dc.subject.keyword | genotipe kentang | id |
| dc.subject.keyword | in vitro | id |
| dc.subject.keyword | keripik kentang | id |
| dc.subject.keyword | transgen | id |
