dc.contributor.advisor | Andrianto, Dimas | |
dc.contributor.advisor | Safithri, Mega | |
dc.contributor.author | Datu'salamah, Wani | |
dc.date.accessioned | 2023-06-21T04:39:38Z | |
dc.date.available | 2023-06-21T04:39:38Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/119762 | |
dc.description.abstract | Sirih merah (Piper crocatum Ruiz & Pav.) memiliki senyawa potensial seperti flavonoid yang dapat berperan sebagai inhibitor tirosinase. Penelitian ini bertujuan untuk menentukan kadar total fenolik dan flavonoid ekstrak sirih merah berdasarkan perbedaan pelarut serta aktivitas inhibisinya terhadap enzim tirosinase. Ekstrak yang mampu menginhibisi enzim tirosinase mampu bekerja sebagai agen pemutih, yang bisa digunakan dalam suatu krim pemutih. Metode ekstraksi yang digunakan yaitu maserasi untuk pelarut etil asetat dan perebusan untuk pelarut air, dilanjutkan dengan uji kadar total fenolik, flavonoid, dan uji inhibisi tirosinase yang berpotensi sebagai agen pemutih. Hasil yang diperoleh pada penelitian ini menunjukkan ekstrak etil asetat menjadi sampel terbaik dengan nilai total fenolik sebesar 56,255 mg GAE/g DW, kadar total flavonoid sebesar 48,631 mg QE/g DW, dan berpotensi sebagai penghambat tirosinase dengan nilai IC50 1600,38 mg/L. Sementara ekstrak air memiliki nilai kadar total fenolik sebesar 44,2 mg GAE/g DW, kadar total flavonoid sebesar 39,812 mg QE/g DW, dan berpotensi sebagai penghambat tirosinase dengan nilai IC50 1764,39 mg/L. | id |
dc.description.abstract | Red betel (Piper crocatum Ruiz & Pav.) is a plant with flavonoid-like potential compound that can act as tyrosinase inhibitor. This research aimed to determine the total phenolic and flavonoid concentration of red betel extract based on different solvents and its inhibition activities against the tyrosinase enzyme. Extract capable of inhibiting the tyrosinase enzyme had potential to act as a whitening agent, which could then be utilized in a whitening cream product. The extraction methods include: maceration for the ethyl acetate solvent; and boiling for the water solvent, continued with measuring the phenolic and flavonoid content, with the tyrosinase inhibition test for solvents with whitening agent potential. Results indicated that the ethyl acetate solvent was the best sample with a total phenolic value of 56,255 mg GAE/g DW, total flavonoid content of 48,631 mg QE/g DW, and the potential to inhibit tyrosinase with an IC50 value of 1600,38 mg/L. Meanwhile, the water extract resulted in a total phenolic value of 44,2 mg GAE/g DW and a total flavonoid content of 39,812 mg QE/g DW, with the potential to inhibit tyrosinase with an IC50 value of 1764,39 mg/L. | id |
dc.description.sponsorship | Penelitian Terapan Kompetitif Nasional (PTKN) Tahun Anggaran 2022 No:3881/IT3.L1/PT.01.03/P/B/2022 an Dr. Mega Safithri, S.Si., M.Si | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Penentuan Total Fenolik dan Flavonoid Ekstrak Etil Asetat dan Ekstrak Air Sirih Merah (Piper crocatum Ruiz & Pav.) serta Inhibisinya Terhadap Enzim Tirosinase | id |
dc.title.alternative | Determination of Total Phenolics and Flavonoids Content of Ethyl Acetate and Water of Red Betel Extract (Piper crocatum Ruiz & Pav.) and Their Inhibition of Tyrosinase Enzyme | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | flavonoid | id |
dc.subject.keyword | phenolic | id |
dc.subject.keyword | red betel | id |
dc.subject.keyword | solvent | id |
dc.subject.keyword | tyrosinase | id |