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dc.contributor.advisorAziz, Sandra Arifin
dc.contributor.advisorMunif, Abdul
dc.contributor.authorIman, Andika Akbarul
dc.date.accessioned2022-03-16T00:03:42Z
dc.date.available2022-03-16T00:03:42Z
dc.date.issued2022
dc.identifier.urihttp://repository.ipb.ac.id/handle/123456789/111363
dc.description.abstractSeledri (Apium graveolens L) termasuk dalam suku Umbelliferae atau Apiaceae (suku adas-adasan) yang berasal dari daerah subtropik Eropa dan Asia yang ditemukan pada ketinggian 900 m dpl. Seledri dapat ditanam di dataran rendah beriklim tropis dengan memberi naungan untuk menahan intensitas sinar matahari tinggi, menjaga kelembaban, dan melindungi air hujan. Bakteri endofit merupakan bakteri yang hidup dan mengolonisasi pada jaringan tanaman tanpa menimbulkan gejala penyakit. Selain berperan dalam memacu pertumbuhan tanaman (plant growth promotion bacteria), juga karena kemampuannya menghasilkan zat pemacu tumbuh, memfiksasi nitrogen, memobilisasi fosfat, bakteri endofit juga dapat berperan dalam kesehatan tanaman. Bakteri endofit memiliki banyak kemampuan di antaranya yaitu mampu menghasilkan hormon Indole Acetic Acid (IAA) yang berperan aktif dalam proses pertumbuhan sel tanaman. Bakteri endofit menghasilkan fitohormon dan senyawa lainnya yang mampu mengatasi stres tanaman sehingga membantu tanaman lebih toleran terhadap lingkungan ekstrem. Selain itu bakteri endofit mampu menghasilkan senyawa metabolit sekunder. Penelitian ini bertujuan untuk meningkatkan produksi dan kandungan flavonoid seledri di dataran rendah dengan aplikasi bakteri endofit. Percobaan ini menggunakan rancangan acak kelompok dengan dua faktor yaitu varietas sebagai faktor pertama dan bakteri endofit sebagai faktor kedua. Varietas yang digunakan terdiri atas 3 taraf yaitu Amigo (tipe dataran tinggi), Aroma (varietas dataran tinggi), dan Summer Green (varietas dataran rendah). Empat isolat bakteri endofit yang digunakan yaitu APE35, BAT, EQ26 dan PTM3 dengan 5 taraf yaitu kontrol, APE35 (tunggal), APE35 dan BAT (kombinasi 2 isolat), APE35, BAT dan EQ26 (kombinasi 3 isolat), dan PTM3 (isolat konsorsium). Percobaan menggunakan rumah plastik UV dan 50% naungan. Aplikasi bakteri endofit dengan penyiraman pada 2 (10 ml), 4 (15 ml), dan 6 (20 ml) minggu setelah tanam (MST). Variabel agronomi yang diamati meliputi tinggi tanaman, diameter tajuk, jumlah tangkai, jumlah anakan, jumlah anak daun dan luas daun. Variabel panen meliputi bobot basah akar, bobot kering akar, bobot basah tajuk, bobot kering tajuk, bobot basah total tanaman, bobot kering total tanaman, kadar air tajuk dan kadar air total tanaman. Variabel fisiologi meliputi konsentrasi flavonoid total, NPK tanaman, antosianin, karoten dan total klorofil. Data dianalisis menggunakan aplikasi R studio. Hasil penelitian menunjukkan bahwa isolat bakteri endofit dapat menghasilkan IAA yang mampu membantu pertumbuhan dan perkembangan tanaman. Varietas dataran tinggi Amigo dan Aroma memiliki kandungan flavonoid total yang nyata lebih tinggi dibandingkan dengan varietas dataran rendah Summer Green karena terjadi cekaman abiotik. Total flavonoid akibat pemberian bakteri endofit APE35 dan PTM3 nyata lebih tinggi dari kontrol dan tidak berbeda dengan endofit dua kombinasi APE35+BAT dan tiga kombinasi APE35+BAT+EQ26. Pemberian bakteri endofit meningkatkan total flavonoid saledri. Interaksi varietas Amigo, Aroma dengan semua pemberian endofit dan Summer Green tanpa bakteri endofit tidak berbeda nyata satu sama lainnya, tetapi berbeda dengan varietas Amigo dan Aroma yang tidak diberikan endofit. Bobot basah total varietas Amigo dan Aroma tanpa bakteri endofit secara nyata lebih rendah dibandingkan dengan varietas lain dengan pemberian bakteri endofit dan varietas Summer Green tanpa bakteri endofit. Varietas Amigo dan Aroma memiliki kandungan flavonoid total yang jauh lebih tinggi dibandingkan dengan Summer Green. Bakteri endofit diperlukan untuk memperbaiki pertumbuhan, meningkatkan produksi dan total flavonoid tanaman seledri yang berasal dari dataran tinggi yang ditanam di dataran rendah, dan meningkatkan pertumbuhan produksi seledri di dataran rendah.id
dc.description.abstractCelery (Apium graveolens L) belongs to the Umbelliferae or Apiaceae tribe originating from the subtropics of Europe and Asia and found at an altitude of 900 m above sea level. Celery can be grown in lowland tropical climates by providing shade to withstand high sunlight intensity retain moisture, and celery plants are less resistant to rainwater. Endophytic bacteria are bacteria that grow and colonize plant tissues. In addition to playing a role in improving plant growth (plant growth promotion rhizobacteria), it is also essential because of its ability to produce growth-promoting substances, fix nitrogen, mobilize phosphate, and play a role in plant health. Endophytic bacteria have many abilities, including producing the hormone Indole Acetic Acid (IAA), which plays an active role in the growth process of plant cells. Endophytic bacteria produce phytohormones. It helps plant growth and plant stress produced by biotic and abiotic factors. Endophytic bacteria can produce biological compounds or secondary metabolites. This study aims to increase lowland celery production and total flavonoid by applying endophytic bacteria. This experiment used a randomized block design with two factors, namely variety as the first factor and endophytic bacteria as the second factor. The variety was consisting of 3 levels of Amigo (highland type), Aroma (highland variety), and Summer Green (lowland variety). Four isolates of endophytic bacteria APE35, BAT, EQ26 and PTM3 were used consisting of 5 levels, namely control, APE35 (single isolate), APE35 and BAT (combination of two isolates), APE35, BAT, and EQ26 (combination of three isolates), and PTM3 (consortium). Experiment using UV plastic housing and 50% para net shade. Application by pouring endophytic bacteria at 2 (10 ml), 4 (15 ml), and 6 (20 ml) weeks after planting (MST). The agronomic variables observed included plant height, crown diameter, number of stalks, number of tillers, number of leaflets, and leaf area. Harvest variables included root wet weight, root dry weight, wet canopy weight, dry canopy weight, total plant wet weight, total plant dry weight, crown moisture content, and total plant moisture content. Physiological variables include total flavonoids, plant NPK, anthocyanins, carotenes, and total chlorophyll. The data were analyzed using the R studio application. The results showed that endophytic bacterial isolates could produce IAA which was able to help plant growth and development. The highland varieties Amigo and Aroma had a significantly higher total flavonoid content than the lowland varieties Summer Green due to abiotic stress. Total flavonoids due to endophytic bacteria APE35 and PTM3 were significantly higher than the control and not different from endophytes with two combinations of APE35+BAT and three combinations of APE35+BAT+EQ26. The application of endophytic bacteria increased the total flavonoid of celery. The interactions of the Amigo, Aroma varieties with all endophytes and Summer Green without endophytic bacteria were not significantly different from each other, but different from the Amigo and Aroma varieties that were not given endophytes. The total wet weight of Amigo and Aroma varieties without endophytic bacteria was significantly lower than other varieties with endophytic bacteria and Summer Green varieties without endophytic bacteria. Amigo and Aroma varieties have a much higher total flavonoid content than Summer Green. Endophytic bacteria are needed to improve growth, increase production and total flavonoids of celery originating from the highlands planted in the lowlands, and increase the growth production of lowland celery.id
dc.language.isoidid
dc.publisherIPB Universityid
dc.subject.ddcflavonoidsid
dc.subject.ddcendophytic bacteriaid
dc.subject.ddcIAA (Indole Acetic Acid)id
dc.subject.ddcsalaryid
dc.titlePeningkatan Produksi dan Flavonoid Tiga Varietas Seledri (Apium graveolens L) dengan Pemberian Bakteri Endofit di Dataran Rendahid
dc.title.alternativeIncreased Production and Flavonoids of Three Varieties of Celery (Apium graveolens L.) by Endophytic Bacteria Application in the Lowlands.id
dc.typeThesisid
dc.subject.keywordflavonoidsid
dc.subject.keywordendophytic bacteriaid
dc.subject.keywordIAA (Indole Acetic Acid)id
dc.subject.keywordsalaryid


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