dc.contributor.advisor | Mubarik, Nisa Rachmania | |
dc.contributor.advisor | Manaf, Lisdar A | |
dc.contributor.author | Ayni, Qurrotu | |
dc.date.accessioned | 2022-01-19T03:34:25Z | |
dc.date.available | 2022-01-19T03:34:25Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/110684 | |
dc.description.abstract | One of the problems in tomato (Solanum lycopersicum L.) farming is the emergence
of wilt disease, which is caused by the fungal pathogen Fusarium sp. Pathogenic fungi
have been controlled by spraying synthetic fungicides. The use of fungicides has a
negative impact on other organisms and the environment. The negative impact of
excessive use of synthetic chemicals can be reduced by using biocontrol agents that
produce antifungal compounds. Efforts to control pathogenic fungi naturally using
rhizosphere bacteria to control the growth of pathogenic fungi on tomato plants
(Solanum lycopersicum L) have not been widely reported. This study aims to select
rhizosphere bacteria from 14 bacterial isolates that have been isolated from tomato
farming which are able to inhibit the growth of Fusarium sp. in vitro and characterize
selected bacteria. The results of the bacterial antagonist test against the fungus Fusarium
sp. with the dual culture method showed that 6 out of 14 isolates were able to inhibit the
growth of the fungus Fusarium sp. Isolates TTSG 2.2, TTSG 2.7, TTSG 3.2, TTSG 3.5,
TTSG 3.6, and TCS 3.1 had inhibitory power of 45%. One of the six isolates of
rhizosphere bacteria, namely TTSG 3.6, showed a negative reaction in the hemolysis
test. These results indicate that the bacterial isolate of TTSG 3.6 has the potential to be
used as a control for Fusarium sp. on tomato plants | id |
dc.description.abstract | Salah satu masalah dalam pertanian tomat (Solanum lycopersicum L.) ialah
munculnya penyakit layu, yang disebabkan oleh cendawan patogen Fusarium sp.
Cendawan patogen selama ini dikendalikan dengan menyemprotkan fungisida
sintetik. Pengunaan fungisida berdampak negatif terhadap organisme lain dan
lingkungan. Dampak negatif dari penggunaan bahan kimia sintetis berlebihan,
dapat dikurangi pengunaannya dengan agens biokontrol yang menghasilkan
senyawa anticendawan. Usaha pengendalian cendawan patogen secara alami
menggunakan bakteri rizosfer sebagai pengendali pertumbuhan cendawan
patogen pada tanaman tomat (Solanum lycopersicum L) masih belum banyak
dilaporkan. Penelitian ini bertujuan untuk menyeleksi bakteri rizosfer dari 14
isolat bakteri yang telah diisolasi dari pertanian tanaman tomat yang mampu
menghambat pertumbuhan Fussarium sp. secara invitro dan melakukan
karakterisasi bakteri terpilih. Hasil uji antagonis bakteri terhadap cendawan
Fusarium oxysporum dengan metode dual culture menunjukkan bahwa sebanyak 6
dari 14 isolat mampu menghambat pertumbuhan cendawan Fusarium sp. Isolat
TTSG 2.2, TTSG 2.7, TTSG 3.2, TTSG 3.5, TTSG 3.6, dan TCS 3.1 memilki daya
hambat 45%. Satu dari enam isolat bakteri rizosfer yaitu TTSG 3.6 menunjukkan
reaksi negatif dalam uji hemolisis. Hasil ini menunjukkan bahwa isolat bakteri
TTSG 3.6 berpotensi digunakan sebagai pengendali Fusarium sp. pada tanaman
tomat | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Seleksi dan Karakterisasi Bakteri Penghambat Pertumbuhan Fusarium sp. Asal Tanaman Tomat (Solanum lycopersicum L.). | id |
dc.title.alternative | Selection and Characterization of Bacteria Inhibiting the Growth of Fusarium sp. Derived from the Tomato Plant (Solanum lycopersicum L.). (Solanum lycopersicum L.). | id |
dc.type | Undergraduate Thesis | id |
dc.subject.keyword | biocontrol | id |
dc.subject.keyword | hemolysis test | id |
dc.subject.keyword | percentage of inhibition | id |
dc.subject.keyword | rhizosphere | id |