Potensi Senyawa Aktif Daun Wungu (Graptophyllum pictum (L.) Griff) terhadap PPARγ dalam Meningkatkan Sensitivitas Insulin In Silico

Abstract

Peroxisome Proliferator-Activated Receptor gamma (PPARγ) merupakan faktor transkripsi yang berperan dalam mengontrol sejumlah ekspresi gen terkait regulasi homeostasis glukosa dan sensitivitas insulin melalui mekanisme agonis parsial dan agonis penuh. Potensi senyawa aktif daun wungu dalam menurunkan glukosa darah belum termanfaatkan dengan baik. Penelitian ini bertujuan memprediksi potensi senyawa aktif daun wungu terhadap PPARγ dalam meningkatkan sensitivitas insulin secara agonis parsial dan agonis penuh melalui interaksi molekulernya. Teknik penambatan molekuler menggunakan Autodock Vina dan visualisasinya menggunakan LigPlot+ dan Pymol. Hasil penelitian menunjukkan bahwa senyawa γ-tokoferol memiliki potensi terbaik terhadap agonis parsial PPARγ berdasarkan energi bebas ikat sebesar -9,9 kkal/mol, interaksi, dan situs aktif pengikatan. Sementara itu, luteolin-7-glukosida memiliki potensi terbaik terhadap agonis penuh PPARγ berdasarkan energi bebas ikat sebesar -8,4 kkal/mol, interaksi, dan situs aktif pengikatan. Hasil penelitian ini, senyawa aktif daun wungu berpotensi agonis PPARγ, baik secara agonis parsial maupun agonis penuh.

Peroxisome Proliferator-Activated Receptor gamma (PPARγ) is a transcription factors that controls the expression of a variety of genes involved in regulation of glucose homeostasis and insulin sensitivity through a partial agonist and a full agonist. The potential of active compounds in Graptophyllum pictum (L.) Griff leaves to reduce blood glucose has not been utilized properly. The aims of this research were to predict the potency of Graptophyllum pictum (L.) Griff leaves active compounds that act as PPARγ partial agonists and PPARγ full agonist to increase insulin sensitization, through molecular interactions. Molecular docking techniques using Autodock Vina and its visualization using LigPlot and Pymol. The results showed that the γ-tokoferol has the best potential against PPARγ partial agonists based on binding free energy -9,9 kcal/mol, interaction, and active binding site. Meanwhile, luteolin-7-glucosides has the best potential against PPARγ full agonists based on binding free energy -8,4 kcal/mol, interaction, and active binding site. The results of this study, the Graptophyllum pictum (L.) Griff leaves active compounds had the potential agonist PPARγ, both partially and fully agonists.