Bioremediasi Lahan Tercemar Profenofos Secara ex-situ dengan Cara Pengomposan
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Pesticide have significantly increased agricultural yields while impacting detrimentally on the food chain of wildlife and human. Composting bioremediation strategy relies on mixing the primary ingredients of composting with contaminated soil, wherein as the compost matures, the pollutant will be degraded by the active microorganisms within the mixture. Composting is a relatively new clean-up strategy and because of this, there are a limited number of studies. This study was carried out to determine the concentration of profenofos decomposition rate of profenofos-contaminated soil composted with agricultural wastes (such as saw dust, carrot leaves, beef manure). The mixed of them was based on C/N ratio 40, 35 and 30. The degradation of profenofos was measured during the composting. The composting with C/N ratio 30 mineralized profenofos rapidly than the others after 28 days of composting (98% of profenofos). Bioremediation could be increased the crop yield compared to unbioremediated soil. Some kinds of bacteria have been isolated from the compost mixture. Three of them were able to grow in solid medium containing 1500 ppm of profenofos. The ability to degradate the profenofos was tested in mineral salt peptone yeast (MSPY) medium by changing from opaque medium to clear medium. It’s ability was indicated by the formation of clear zones surrounding the bacteria.