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Title: | Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16 |
Authors: | Meryandin, Anja Hendarwin, Trio Fahrruroz Akhdiya, Alina Saprudin, Deden Lestari, Yulin |
Issue Date: | 2009 |
Publisher: | Biology Department, Faculty of Mathematics and Natural Sciences, Sebelas Maret University Surakarta and Society for Indonesian Biodiversity |
Series/Report no.: | Volume 9, Number 3; |
Abstract: | Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90°C. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2 % hours at 90°C. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1,98 mg/ml and 523 μmoVminute/mg, respectively Key words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces. |
URI: | http://repository.ipb.ac.id/handle/123456789/76291 |
ISSN: | 1412-033X |
Appears in Collections: | Faculty of Mathematics and Natural Sciences |
Files in This Item:
File | Size | Format | |
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ART2009ame.pdf | 2.09 MB | Adobe PDF | View/Open |
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