Characterization and Purification a Specific Xylanase Showing Arabinofuranosidase Activity from Streptomyces spp. 234P-16

Abstract

Streptomyces spp 234P-16 producing xylanase was isolated from soil sample from Padang, West Sumatra, Indonesia. Crude enzyme (produced by centrifuging the culture at 14000 rpm for about 5 minutes) and purified xylanase have an optimum condition at pH 5 and 90°C. Crude xylanase have half life time of 4 hours, whereas purified xylanase have half life time of 2 % hours at 90°C. The molecular mass of purified xylanase was determined to be 42.4 kDa. The Arabinofuranosidase have a Km and Vmax value of 1,98 mg/ml and 523 μmoVminute/mg, respectively Key words: xylanase, xylooligosaccharide, arabinofuranosidase, Streptomyces.