Pengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC)

Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/24742

Title:	Pengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC)
Other Titles:	Development Of Method For Production Of Escherichia Coli Enteropathogenic (EPEC) Antigen
Authors:	Martini S. Nurhayati, Tati Purwanto S.B. Wibawan, I Wayan Teguh
Issue Date:	2005
Publisher:	IPB (Bogor Agricultural University)
Abstract:	A study was carried out to develop a method of enteropathogenic Escherichia coli (EPEC) protease antigen production. In the first trial EPEC strain Kl .1 which was cultured in the LCT-skimmilk- starch medium to produce protease. After purification using centricon this antigen induced non specific polyclonal antibody and cross reacted with the production medium. On the other hand a specific reaction was found from that of the bacteria grown in minimal medium M9 and precipitated with 45% ammonium sulphate. The protease had molecular weight of approximately 58.6 kDa and 37.4 kda with specific protease activity 0.019 1UI mg protein. Key words: enteropathogenic, E. coli, (EPEC), protease enzym, polyclonal antibody
URI:	http://repository.ipb.ac.id/handle/123456789/24742
Appears in Collections:	Animal Disease and Veterinary Health

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pengembangan metode produksi ..(5 hal).pdf	Publikasi	210.51 kB	Adobe PDF	View/Open
pengembangan metode produksi ..(5 hal).ps	Postscript	346.22 kB	Postscript	View/Open

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