Pengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC)

Abstract

A study was carried out to develop a method of enteropathogenic Escherichia coli (EPEC) protease antigen production. In the first trial EPEC strain Kl .1 which was cultured in the LCT-skimmilk- starch medium to produce protease. After purification using centricon this antigen induced non specific polyclonal antibody and cross reacted with the production medium. On the other hand a specific reaction was found from that of the bacteria grown in minimal medium M9 and precipitated with 45% ammonium sulphate. The protease had molecular weight of approximately 58.6 kDa and 37.4 kda with specific protease activity 0.019 1UI mg protein. Key words: enteropathogenic, E. coli, (EPEC), protease enzym, polyclonal antibody