Pengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC)

Please use this identifier to cite or link to this item: http://repository.ipb.ac.id/handle/123456789/24742

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DC Field	Value	Language
dc.contributor.author	Martini S.
dc.contributor.author	Nurhayati, Tati
dc.contributor.author	Purwanto S.B.
dc.contributor.author	Wibawan, I Wayan Teguh
dc.date.accessioned	2010-05-24T03:41:01Z
dc.date.available	2010-05-24T03:41:01Z
dc.date.issued	2005
dc.identifier.uri	http://repository.ipb.ac.id/handle/123456789/24742
dc.description.abstract	A study was carried out to develop a method of enteropathogenic Escherichia coli (EPEC) protease antigen production. In the first trial EPEC strain Kl .1 which was cultured in the LCT-skimmilk- starch medium to produce protease. After purification using centricon this antigen induced non specific polyclonal antibody and cross reacted with the production medium. On the other hand a specific reaction was found from that of the bacteria grown in minimal medium M9 and precipitated with 45% ammonium sulphate. The protease had molecular weight of approximately 58.6 kDa and 37.4 kda with specific protease activity 0.019 1UI mg protein. Key words: enteropathogenic, E. coli, (EPEC), protease enzym, polyclonal antibody	id
dc.publisher	IPB (Bogor Agricultural University)
dc.title	Pengembangan Metode Produksl Antigen Protease Escherichia Coli Enteropatogenik (EPEC)	id
dc.title.alternative	Development Of Method For Production Of Escherichia Coli Enteropathogenic (EPEC) Antigen	id
Appears in Collections:	Animal Disease and Veterinary Health

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pengembangan metode produksi ..(5 hal).pdf	Publikasi	210.51 kB	Adobe PDF	View/Open
pengembangan metode produksi ..(5 hal).ps	Postscript	346.22 kB	Postscript	View/Open

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