Please use this identifier to cite or link to this item:
http://repository.ipb.ac.id/handle/123456789/132452
Title: | Aktivitas Antioksidan In Vitro dan Regulasi Persinyalan Antioksidan In Silico oleh Ekstrak Larva Black Soldier Fly (Hermetia illucens) |
Other Titles: | In Vitro Antioxidant Activity and In Silico Antioxidant Signaling Regulation by Black Soldier Fly (Hermetia illucens) Larvae Extract |
Authors: | Sulistiyani, Sulistiyani Setyawati, Inda Nurinayah, Irna |
Issue Date: | 2023 |
Abstract: | Stres oksidatif di dalam tubuh dapat diatasi dengan menginhibisi protein
Keap1 yang berasosiasi dengan Nrf2 dalam mekanisme persinyalan antioksidan.
Penelitian bertujuan mengidentifikasi senyawa aktif ekstrak larva BSF yang
dibudidayakan dengan substrat limbah dapur di Desa Sindangjaya, Cianjur
sebagai antioksidan melalui pengujian DPPH dan prediksi in silico. Ekstraksi
dilakukan menggunakan metode maserasi bertingkat dengan pelarut aseton dan
metanol serta dengan maserasi pemanasan menggunakan pelarut air, kemudian
dianalisis dengan LC-MS/MS dan diuji DPPH. Potensinya sebagai inhibitor
Keap1 secara in silico dengan uji bioavailabilitas, ADME, toksisitas, dan
penapisan virtual. Senyawa aktif 1,5-Bis(1H-benzotriazol-1-yl)-1,5-pentanedione
di dalam ekstrak air dari golongan benzotriazoles memiliki nilai energi afinitas
yaitu -7,01 kkal/mol dan Methyl (S)-2-((S)-2-((tert-butoxycarbonyl) amino)-3
cyclohexylpropanamido)-3-((S)-2-oxopyrrolidin-3-yl) propanoate di dalam
ekstrak aseton dari golongan asam amino, peptida, dan turunanya memiliki nilai
energi afinitas sebesar -6,49 kkal/mol. Kedua senyawa tersebut merupakan
kandidat terbaik sebagai inhibitor Keap1 dalam persinyalan antioksidan karena
berinteraksi dengan residu Cys151. Oxidative stress in the body can be reduced by inhibiting the protein Keap1 from associating with Nrf2 in an antioxidant signaling mechanism. The study aimed to identify active compounds of BSF larvae extract cultivated with kitchen waste substrate in Sindangjaya Village, Cianjur as antioxidants with DPPH method and in silico prediction. Extraction was carried out using a multilevel maceration method with acetone and methanol solvents and followed by heated maceration with aqueous solvent. The extracts were analysed using LC-MS/MS method and DPPH test. Its potential as Keap1 inhibitor was predicted using in silico bioavailability test, ADME, toxicity, and virtual screening. The active compound of 1,5-Bis(1H-benzotriazol-1-yl)-1,5-pentanedione of the aqueous extract which is benzotriazoles group has an affinity energy value of -7,01 kcal/mol and Methyl (S)-2-((S)-2-((tert-butoxycarbonyl) amino)-3 cyclohexylpropanamido)-3-((S)-2-oxopyrrolidin-3-yl) propanoate in the acetone extract from the amino acid, peptides, and derivatives group has an affinity energy value of -6,49 kcal/mol. Both compounds are the best candidates for Keap1 inhibitors in antioxidant signaling due to their interaction with the Cys151 residue. |
URI: | http://repository.ipb.ac.id/handle/123456789/132452 |
Appears in Collections: | UT - Biochemistry |
Files in This Item:
File | Description | Size | Format | |
---|---|---|---|---|
01 Watermark (Cover) - SKRIPSI - (G84190020) IRNA NURINAYAH.pdf Restricted Access | Cover | 362.8 kB | Adobe PDF | View/Open |
02 Watermark (Fullteks) - SKRIPSI - (G84190020) IRNA NURINAYAH.pdf Restricted Access | Full teks | 1.57 MB | Adobe PDF | View/Open |
03 Watermark (Lampiran) - SKRIPSI - (G84190020) IRNA NURINAYAH.pdf Restricted Access | Lampiran | 651.12 kB | Adobe PDF | View/Open |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.