| dc.description.abstract | Silkworm diseases constitute a major constraint in sericulture productivity. The purposes of this research were to isolated entomopathogenic microorganism (MEP) from soil and mulberry leaves, to study the potency of MEP to infect larvae and their effect to cocoon quality. Various patho genic microbes have been isolated from mulberry leaves and soil i.e. nematodes Steinernematidae, bacteria isolate PT, KP and PB from soil and bacteria isolate PT and KC from mulberry leaves. The entomopathogenic fungi isolated from soil were Beauveria sp., Penicillium sp. and Metarhizium sp., from mulberry leaves were Cladosporium sp., Aspergillus sp. (yellow) (As1) and Aspergillus sp. (violet) (As2). Soil was as potensial as mulberry leaves in provoking disease to larvae, fungi has potential better than bacteria and nematodes. In the pathogenicity test of various isolates, entomopathogenic nematodes (Steinernematidae) isolated from soil caused larval mortality 46%, increased the percentage abnormal cocoon (29,85%), reduced single cocoon weight (1,73 gram) and decreased cocoon shell weight (19,78%). The dark yellow bacteria (KP) isolated from soil reduced the single cocoon weight (1,44 gram) and the light yellow bacteria (KC) isolated from mulberry leaves the decrease cocoon shell weight percentage (19,17%). Aspergillus sp. violet (As2) isolated from mulberry leaves caused larva mortality 60,00%, Aspergillus sp. yellow (As1) isolated from mulberry leaves caused high abnormal cocoon (32,28%). | id |
