Peranan Zat Ekstraktif Dalam Pembentukan Gaharu Pada Aquilaria crassna Pierre ex Lecomte dan Aquilaria microcarpa Baill
Abstract
This research is aimed at investigating the anti fungal activity (AFA), the correlation of gaharu tree’s phenolics in relation with the susceptibility of the trees to Fusarium bulbigenum attack in the formation of agarwood, and to observe total phenolics content and tree’s chemicals of Aquilaria crassna and Aquilaria microcarpa pre-inoculation, the reology of tree’s chemical and microorganism change along inoculation time. There were variations of anti fungal activity among trees, and the highest is at tree no. 10 that AFA was in the middle class (39,77%), but entirely agarwood trees exhibited a low anti fungal activity at lab scale. Further fractionation and bioassay showed that the most active component are likely in the ethyl acetate fraction that exhibited strong anti fungal activity at 4% concentration. Trees as many as 20 of A. microcarpa at Carita and 10 of A. crassna at Darmaga were inoculated with isolate of F.bulbigenum in spiral pattern around the stem. Prior to inoculation, strips were taken from the stem for total phenolics content and chemicals analyzing. The result revealed that total phenolics content and infection area exhibited a negative correlation, which means the higher the phenolics content, the smaller the infection area. The preinoculation chemicals analysis showed that tree with smaller infection area had larger type of compounds and higher concentration of phenolics than tree with wider one. Some chemicals that at certain plants acted as defense system, according to some references, were found at higher concentration in the gaharu trees with smaller infection or they were not even traced in other tree. The reology analysis revealed that the largest types of compounds were noticed at three day’s old inoculation. Agarwood constituents and other aromatic compounds were detected since three days after inoculation which is indicated the agarwood formation was taken place. Meanwhile, the microbiology analysis showed there was no microbes change in the inoculation sites along 6 month inoculation time, but some small insects, such as ants and bugs, were found at the area.
Collections
- MT - Forestry [1412]