Pemurnian dan Karakterisasi Protease Ekstraseluler Isolat Bakteri Termofilik GP-04
Purification and Characterization of Extracellular Proteases from Isolate GP-04, a Thermophilic Bacterium.
| dc.contributor.advisor | Suhartono, Maggy Thenawidjaja | |
| dc.contributor.advisor | Suwanto, Antonius | |
| dc.contributor.advisor | Santosa, Dwi Andreas | |
| dc.contributor.advisor | Guhardja, Edi | |
| dc.contributor.author | Mubarik, Nisa Rachmania | |
| dc.date.accessioned | 2010-03-24T01:51:16Z | |
| dc.date.available | 2010-03-24T01:51:16Z | |
| dc.date.issued | 2001 | |
| dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/848 | |
| dc.description.abstract | A gram positive, rod-shaped, and aerobic thermophilic bacterium, designated as GP-04, was isolated from Gunung Pancar hot spring, near Bogor, West Java. Based on approximately 400 bases both 5 frames regions and 3 frames ends DNA sequences of 16S-rRNA gene, the isolate is determined to be closely related (+ 90%) to Bacillus caldovelox. The isolate, produced significant amount of extracellular protease when grown in a modified Thermophilic Bacillus medium pH 7,0 at 65°C for 16 h in a batch mode fermentation. Crude protease was purified using 30% ammonium sulphate precipitation and column chromatography using Sephadex G-25 followed by DEAE-Sephadex A-50. | en |
| dc.publisher | IPB (Bogor Agricultural University) | |
| dc.subject | Bogor Agricultural University (IPB) | id |
| dc.title | Pemurnian dan Karakterisasi Protease Ekstraseluler Isolat Bakteri Termofilik GP-04 | id |
| dc.title | Purification and Characterization of Extracellular Proteases from Isolate GP-04, a Thermophilic Bacterium. | en |
