Karakteristik dan Status Akrosom Spermatozoa Domba (Ovis aries) yang Dibekukan dalam Pengencer Tris Kuning Telur atau Tris Susu Skim dengan Metode One atau Two Step.

Abstract

This research was conducted to study characteristics (motility, viability, plasma membrane integrity) and intact acrosome status of ram’s spermatozoa cryopreserved in tris-egg yolk or tris-skim milk extenders with one or two step method. Semen was collected from local ram, devided and extended in tris-egg yolk or tris-skim milk by using one step method (OS-KT and OS-SK) and two step method (TS-KT and TS-SK). The results showed that equilibrated spermatozoa had similar quality in all groups (P>0.05). Percentage of post-tahwed motility spermatozoa in OS-SK group was lower than OS-KT group (P<0.05). Percentage of spermatozoa viability, plasma membrane integrity and intact acrosome status were not significantly different among groups (P>0.05). In conclusion, tris-egg yolk extender was better than tris-skim milk extender to prevented the motility of spermatozoa after cryopreservation. Futhermore, one or two step methods were not significantly effected to the quality of post-thawing spermatozoa.