IDENTIFIKASI KERAGAMAN GEN GH, GHRH, DAN PIT-1 PADA KERBAU DI PROVINSI BANTEN

Abstract

The objectives of this study were to get information of genetic variation of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. Three loci of growth hormone genes (GH|MspI, GHRH|HaeIII and Pit-1|HinfI) were used on molecular polymorphism study. Molecular analysis showed that GH|MspI and GHRH|HaeIII loci of Banten population were polymorphic, while Pit-1|HinfI locus was monomorphic. Genetic polymorphisms of GH|MspI locus was low showed by expected heterosigosity value (He = 0.0469). While, genetic polymorphisms of GHRH|HaeIII locus was higher (He = 0.4908). FIS index showed negative value indicated that there was a random mating system in Banten buffalo population. FIT value for GH|MspI locus near to 0 (- 0.0207) indicated that there was a balanced population according to Hardy-Weinberg principle. A bias of Hardy Weinberg principle was on GHRH|HaeIII locus showed by FIT value near to -1 (- 0.7224). Population differentiation indicator, FST index showed a small value (0.0024) indicated that differentiation of Banten population to two subpopulations (Pandeglang and Lebak) only decreased a small number of genetic diversity (0.24%).