IDENTIFIKASI KERAGAMAN GEN GH, GHRH, DAN PIT-1 PADA KERBAU DI PROVINSI BANTEN
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The objectives of this study were to get information of genetic variation of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. Three loci of growth hormone genes (GH|MspI, GHRH|HaeIII and Pit-1|HinfI) were used on molecular polymorphism study. Molecular analysis showed that GH|MspI and GHRH|HaeIII loci of Banten population were polymorphic, while Pit-1|HinfI locus was monomorphic. Genetic polymorphisms of GH|MspI locus was low showed by expected heterosigosity value (He = 0.0469). While, genetic polymorphisms of GHRH|HaeIII locus was higher (He = 0.4908). FIS index showed negative value indicated that there was a random mating system in Banten buffalo population. FIT value for GH|MspI locus near to 0 (- 0.0207) indicated that there was a balanced population according to Hardy-Weinberg principle. A bias of Hardy Weinberg principle was on GHRH|HaeIII locus showed by FIT value near to -1 (- 0.7224). Population differentiation indicator, FST index showed a small value (0.0024) indicated that differentiation of Banten population to two subpopulations (Pandeglang and Lebak) only decreased a small number of genetic diversity (0.24%).
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