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      Pengaruh jenis inokulum rhizopus oligosporus dan rhizopus oryzae terhadap sifat fisiko-kimia tempe kacang merah

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      Date
      2013
      Author
      Firlieyanti, Antung Sima
      Purnomo, Eko Hari
      Kusnandar, Feri
      Maknun, Lulu
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      Abstract
      Tempe kacang merah diketahui memiliki kadar protein yang lebih rendah dibandingkan tempe kedelai. Upaya meningkatkan kadar protein dapat dilakukan melalui pendekatan optimasi kondisi fermentasi, antara lain jenis kapang dan waktu fermentasi. Tempe umumnya diproduksi dengan menggunakan kapang R. oligosporus sebagai kultur utama. Aplikasi R. oligosporus dan R. oryzae secara tunggal atau kombinasi diduga dapat menghasilkan tempe dengan karakteristik yang berbeda. Penelitian ini bertujuan untuk mempelajari pengaruh komposisi inokulum (laru R. oligosporus, laru R. oryzae, laru R. oligosporus+R. oryzae, dan laru R. oligosporus + laru R. oryzae) dan waktu inkubasi (24, 36, dan 48 jam) terhadap sifat fisik dan kimia tempe kacang merah, terutama pengaruhnya terhadap peningkatan kadar protein tempe kacang merah. Kombinasi kapang R. oligosporus + R. oryzae (laru K1+K2) mampu menghasilkan tempe kacang merah dengan karakteristik yang lebih baik dibandingkan aplikasi kapang secara tunggal. Aplikasi laru K1+K2 menghasilkan tempe dengan miselium yang kompak dengan waktu fermentasi yang lebih cepat (24–36 jam) dibandingkan laru K2. Tempe laru K1+K2 juga memiliki kadar protein terlarut unggul dalam parameter kadar protein terlarut dibandingkan tempe laru K1.
       
      Red kidney bean tempeh has been reported to contain less protein content as compared to soy bean tempeh. It is believed that protein content of tempeh depends not only on the protein content of the raw material but also processing parameters i.e. inoculum and fermentation time. Therefore, the objective of this research was to study the effect of different inoculum and fermentation time on the physicochemical properties of red kidney bean tempeh. Single and combined application of two types of mold normally used for tempeh fermentation (R. oligosporus and R. oryzae) and three different fermentation times (24, 36, and 48 hours) were studied. Mixed-culture inoculum of R. oligosporus and R. oryzae produces tempeh with improved physicochemical properties compared to their single application. Tempeh with mixed-culture inoculum shows good growth of mycelium and requires shorter fermentation time (24–36 hours), as compared to tempeh with R. oryzae inoculum. The tempeh also has higher soluble protein content (21,01%) than tempeh made by inoculum of R. oligosporus.
       
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      http://repository.ipb.ac.id/handle/123456789/71989
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      • Research Proceeding [307]

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      Copyright © 2020 Library of IPB University
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      Contact Us | Send Feedback
      Indonesia DSpace Group 
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      Universitas Jember Digital Repository