Fraksi Aktif Antioksidan dari Ekstrak Kulit Kayu Sungkai (Peronema canescens Jack.)

Abstract

Sungkai (Peronema canescens Jack.) bark extract is potentially containing extractive materials with antioxidant properties. The objective of this research was to fractionate 70% of ethanol extract of sungkai bark, hydrolize the n-hexane residue, determine fraction content and antioxidant activity using in vitro testing, and analyze chemical compounds found in three of the most active fraction. Fractionation was conducted using solvent-solvent extraction with n-hexane and ethyl acetate. Antioxidant activity was examined using 1,1-difenil-2-pikrilhidrazil (DPPH) method. The chemical components of three most active fraction were analyzed by gas chromatography mass spectrometry (GC-MS). Extract value of n-hexane fraction was the highest levels.. The three most active fractions with antioxidant properties are non-hydrolized etil acetate, n-hexane, and hydrolized ethyl acetate (IC50 43.67, 44.55, and 53.34 μg/mL). Phenolic compound identification shows several dominant compounds: quinic acid, guaiacol, hydroquinone, isovanillic acid, genkwanin, catechol, and benzoic acid. Those compounds are categorized as phenol and own strong antioxidant activity