Difference effects of tempe and tofu flours on serum estrogen, serum amyloid beta as well as on cognitive function of ovariectomized female rats
Perbedaan pengaruh tepung tempe dan tepung tahu terhadap estrogen serum, beta amiloid serum, dan fungsi kognitif pada tikus betina dengan ovariektomi
Damanik, M. Rizal M.
Rahardjo, Tri Budi W.
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The aims of this study were to measure the content of isoflavone, vitamin B6, vitamin B12, folic acid, and protein in tempe and tofu flours, to analized the effect of tempe and tofu flours on serum estrogen and amyloid beta level, and cognitive function in ovariectomized female rats. Seventy two (72) white female Sprague Dawley strain rats, aged 12 months use for this study. Before the intervention all rats had ovariectomy (OVx) surgical treatments and were grouped into 5 intervention groups (each 4 rats): tempe flour, tofu flour, estradiol, casein; and non ovariectomy. Analysis of estrogen serum is using Elisa Reagents Estradiol/ ELISA EIA-2693. Analysis of serum beta amyloid by Elisa Reagent Amyloid Beta Peptide 1-40 (AB1-40) (E90864Ra). Cognitive function was measured using a labyrin. Processing and data analysis, to see the average and the average difference between the treatment groups used One-Way ANOVA with Polynomial Contrass Post Hoc LSD. Analysis of the correlation between variables is done using correlation test. Analysis of the variables that influence cognitive function by Multiple Linear Regression. The results showed that isoflavone content (genestein) in tempe flour (50.558 mg/100 g) two times higher than tofu flour (19.923 mg/100 g). Nutrient content (vitamin B6, vitamin B12, folic acid, and protein) of tempe flour more higher than tofu flour. Nutrient content in soybean flour as follows: vitamin B6 (0.28 mg/100 g), vitamin B12 (0.97 mcg/100 g), folic acid (0.0251 mg/100 g). Nutrient content of tofu flour is vitamin B6 (0.19 mg/100g). Tofu flour not contains vitamin B12 and folic acid. Protein of tempe flour content (41.5%) lower than the tofu (47.4%). Tempe flour group had a significant (P<0.05) increase compared to after OVx (baseline 2), while the increase in serum estrogen of tofu flour group were not significant (P>0.05). Tempe flour group compared with baseline 2 showed a significant decrease (P<0.05), while in the tofu flour group was not significant (P>0.05). Based on the difference test between the tempe flour group with baseline 2 show an increase score in cognitive function significantly (P<0.05), while the tofu group not significantly different (P>0.05). Multiple Linear Regression test results showed that the most variables associated with cognitive function is amyloid beta (P<0.05). Conclusions this study is the content of nutrients and isoflavones (genistein) on tempe flour higher than tofu flour. Intervention by tempe flour can increase serum estrogen and may decrease beta amyloid, abdominal fat and body weight in female rats with ovariectomy. Beta amyloid serum is the variable most associated with cognitive function. Suggested for further research is to measure cognitive function of other aspects such as memory, the content of serotonin, dopamine, plaque on brain.
- DT - Human Ecology