Evaluasi keragaman genetik gen hormon pertumbuhan (GH) pada sapi pesisir Sumatera Barat menggunakan penciri PCR-RFLP

Abstract

A total of 134 Pesisir cattle were genotyped for growth hormone (GH) gene by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). The genotype and allele frequencies of the GH Mspl and GH AluI Pesisir cattle were determined. The GH Mspl gene frequencies for the C and T allele were 0.209 and 0.791 respectively, while GH AluI gene frequencies for the L and V allele were 0.992 and 0.008 respectively. The chi-square analysis indicated that this population is not in Hardy-Weinberg Equilibrium status. Expected heterozygosis value (He) for GH Mspl and GH AluI were 0.3306±0.0266 and 0.0149±0.0073 respectively. The PCR-RFLP AluI marker. This finding showed that GH Mspl T allele was favorable as a GH marker for Bos indicus breeds.