Diferensial Leukosit dan Rasio Neutrofil/Limfosit pada Kerbau Lumpur (Bubalus bubalis) Betina.

Abstract

The goal of this research was to study the total number of leucocytes and its variant of leucocytes, also neutrophil/lymphocyte (N/L) ratio as stress index of four female swamp buffaloes for ten weeks. Blood sampling was done every two days for ten weeks. Blood samples were collected from jugularis vein by using an EDTA coated tube. White blood cells was counted on hemocytometer and the blood smears were stained by Giemsa to count the number of total variant of leukocytes. The average of total number of leucocyte was 10845,89±2518,20 cells/mm3, neutrophil was 6084,29±2214,96 cells/mm3, eosinophil was 170,88±156,77 cells/mm3, monocyte was 231,65±172,89 cells/mm3, dan lymphocyte was 4361,17±1926,58 cells/mm3. Neutrophil/lymphocyte (N/L) ratio was 1,76±1,13

Penelitian ini dilakukan untuk mengetahui jumlah total leukosit, jenis-jenis leukosit, dan rasio neutrofil/limfosit (N/L) sebagai indeks stres pada kerbau lumpur (Bubalus bubalis) betina selama sepuluh minggu. Darah kerbau lumpur diambil setiap dua hari sekali selama sepuluh minggu. Darah diambil melalui vena jugularis menggunakan tabung berisi EDTA. Sel darah putih dihitung dengan kamar hitung (hemocytometer) dan preparat ulas darah untuk menghitung jenis leukosit diwarnai dengan pewarnaan giemsa. Rataan dari total leukosit adalah 10845,89±2518,20 sel/mm3 , dengan total neutrofil 6084,29±2214,96 sel/mm3, eosinofil 170,88±156,77 sel/mm3 , monosit 231,65±172,89 sel/mm3, dan limfosit 4361,17±1926,58 sel/mm3. Rasio N/L pada kerbau 1,76±1,13.