Aktivitas oksidasi amonium dan reduksi Nitrat Alcaligenes sp. Isolat SDTT5 dan KDTST3 asal tambak udang menggunakan berbagai sumber karbon

Abstract

Water quality is one problem found in shrimp culture caused by increasing of ammonium and nitrite concentration. This problem correlates with a~mulation of uneaten feed and shrimp waste products. Both ammonium and dhUte are toxic to shrimp. Recently, addition of bacteria as bioremidiation agent to;:shrimp ponds can remedy water quality. SDTT5 and KDTST3 isolate are ni:trate reducing bacteria isolated from shrimp ponds using denitrification media ~h acetate as carbon source in anaerob condition. Based on mollecular analysis 0~1 6S-rRNA sequences the isolate was 99% closely related to Alcaligenes sp. TIDs study was to investigate ammonium oxidation and nitrate reduction activities bis DTT5 and KDTST3 isolates on different carbon sources. This expriment was c<j.ducted used different media i.e. heterotroph and autotroph nitrification media, ami ammonium oxidation and nitrate reduction media. The incubation was held u~er either aerob or anaerob conditions. The results shows that SDTT5 and Kf>TST3 isolates were able to oxidize ammonium heterotrophycally with acetate, s~cinate, glucose and glycerol as carbon sources and autothrophically with cibonate as carbon source. The highest activity of ammonium oxidation by St>TT5 and KDTST3 was found in succinate and acetate as carbon sources. S~TT5 isolate was able to oxidize ammonium and reduce nitrate either aerobically or anaerobically with different carbon sources. Under anaerob c.gndition SDTT5 isolate was able to oxidize ammonium and reduce nitrate with a~tate, succinate, glucose and glycerol as carbon sources but SDTT5 isolate can not oxidize ammonium and reduce nitrate aerobically with glycerol as carbon source Meanwhile, KDTST3 isolate was only able to oxidize ammonium aerobically with acetate, succinate, and glucose as carbon sources, and reduced nitrate with glycerol as carbon source.