Browsing Faculty of Animal Science by Author "Otoi, T."
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Effects of Centrifugation and Lipid Removal on the Cryopreservation of in Vitro Produced Bovine Embryos at the Eight-Cell Stag
Murakami, M. | Otoi, T. | Sumantri, Cece | Suzuki, T. (1998)The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes ... -
Normal Calves Obtained after Direct Transfer of Vitrified Bovine Embryos Using Ethylene Glycol, Trehalose, and Polyvinylpyrrolidone
Saha, S. | Otoi, T. | Takagi, M. | Boediono, Arief | Sumantri, Cece | Suzuki, T. (1996)In the present study, IVF bovine embryos were vitrified using as the cryoprotectants, ethylene glycol plus trehalose plus the polymer, polyvinylpyrrolidone (PVP). In Experiment I, toxicity of the vitrification solution ...