Pregnancies from obstructive azoospermia patients after intracytoplasmic sperm injection (ICSI) with testicular spermatozoa
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The success rate of ICSI in tenns of fertilization rate and resulting pregnancies using testicular spem1 was evaluated. Testicular biopsy was perfom1ed on t he hushands and the tissue was dissected followed by washing twice in modified human tuhal fluid medium. Ovarian stimulation wac; done by injection uf hMG after down regulation using GnRH analogue. The ICSI procedure was atrried out with fn-sh testicular spcnnatozoa following hy cultured in vitro of the zygote. •n,e average age of the wives treated was 34 years old. The number of oocytes retrieved was I 40 oocytcs of which 115 (t)2%) oocytc.'i in metaphase II stage. Fertilization rate of ICS l with testicular spem1 was 61% (70/115) with four zygotes showing three pronuclei. One to three of the hest developed embryos were trunsferred to the mothers on day two or three and resulted on 9 out off 17 (53%) patients were pregnant. ICSJ elf testicular sperm is a u•seful treatment for patient with uhstructive azoospermia 111 whom standard microsurgical procedures ure non feasible.
- Faculty of Veterinary