Studi keragaman fenotipik dan genetik beberapa sub populasi kambing lokal Indonesia dan strategi pemanfaatannya secara berkelanjutan

Abstract

The genetic resources of local goats are very important because it was well adapted to the local environment with low input management systems. Local goats are very potential for breeding stock program which suitable to the Indonesia local conditions. The study was carried out in order to describe the phenotypic and genetic of 6 sub-populations of Indonesian local goats based on the analysis of the morphometrics, mitochondrial DNA, Y Chromosome and GDF9 gene. The sampling was conducted in four of the Provinces in Indonesia, namely North Sumatra Province (Samosir goat n=42 in the Samosir District, Muara goat n=34 in the North Tapanuli district, Kacang goat n=217 in the Deli Serdang District), Central Java Province (Jawarandu goat n=94 in the Blora District); Province of South Sulawesi (Marica goat n=60 in the Maros District, Makassar City, Jeneponto District) and Nusa Tenggara Timur Province (Bengala goat n=96 in the Kupang area, Sikka District, Ende District). All of them were measured for morphometric and was bleeding to collect blood as DNA sources. The blood sample was preserve with absolute ethanol. DNA extracted was analyzed by PCR-RFLP methods for D-loop region, SRY gene and GDF gene on Indonesian local goat does.