Efektifitas Kunyit, Bawang Putih, Dan Zink Dalam Pakan Terhadap Aktivitas Dan Kapasitas Fagositosis Sel Polimorfonuklear Ayam Broiler
Abstract
This research was aimed to defined the effect of turmeric, garlic, and zinc to broiler chicken polymorphonuclear (PMN) cell's phagocytosis activity and capacity. A hundred broiler chicken were divided into 5 treatments: RO (basale feed), R1 (basale feed, garlic 2.5%, turmeric 1.5%), R2 (basale feed, garlic 2.5%, ZnO 120 ppm), R3 (basale feed, turmeric 1.5%, ZnO 120 ppm), and R4 (basale feed, garlic 2.5%, turnleric 1.5%, ZnO 120 ppm) each treatment consist of 4 repetitions. The blood samples were collected from 3 weeks and 6 weeks old healthy broiler chicken. The blood samples challenged in vitro with Escherichia coli (10' cfdml). The blood smear then stained using Giemsa staining technique and observed with light microscope, magnified 100 times. Phagocytosis activity measured by counting the cells that engulf the bacterium actively from 100 PMN cells. Phagocytosis capacity measured by counting the bacterium that engulfed by 50 active PMN cells. The result of this research showed that the phagocytosis activity and capacity in broiler chicken at the age 3 and 6 weeks old were insignificant (P0.05). Phagocytosis activity at the age 3 weeks were ranged between 63.56% to 93.2%. Phagocytosis activity when 6 weeks old were ranged between 57.88% and 91.64%. The average of phagocytosis activity of treatment's groups without observed the broiler's age showed that turmeric 1.5% and ZnO 120 ppm addition (R3) gave the better result than the other. Phagocytosis capacity of 3 weeks old broiler chicken were ranged between 178 to 379.01 bacterid50 cells and when 6 weeks old ranged from 292.98 to 483.89 bacterid50 cells. The phagocytosis capacity's average of treatment's groups without observed the broiler's age showed that garlic 2.5% and ZnO 120 ppm addition (R2) were better than the other groups. Phagocytosis activity from all groups insignificantly (P>0.05) decreased at 6 weeks old. The phagocytosis capacity of control group were increased significantly (P<0.05) at 6 weeks old whereas the other treatment groups (Rl, R2, R3, R4) were increased insignificantly (P>0.05).