Angka Konsepsi Hasil Inseminasi Semen Cair Versus Semen Beku Pada Kuda Yang Disinkronisasikan Estus Dan Ovulasi

Abstract

Semen used for arti-kcial insemination (Al) can be prepared in different ways, fresh extended fresh or chilled, or frozen-thawed (FT). The aim of the experiment was to evaluate the use of preserved semen to inseminate the oestrus mares. Nineteen mares were used in this research. The mares were synchronized with doubIe injection of PGF,a 14 days apart. The follicle size was monitored using ultrasound scanner during the third day of oestrus, and 2500 1U hCG was administered at the same time. The A1 was conducted 35 hours after hCG injection with total motile sperm 200x106 for chilled semen and 250-300x106 for frozen semen, The result demonstrated that the response of the oestrus with double injection of PGF,a was 73.7%. The conception rate (CR) was 14.3% (117) with frozen semen and 42.9% (317) with chilled semen. It is concluded that A1 with chilled semen resulted higher conception rate than frozen semen.