Pengaruh Penambahan Antibiotika pada Biosintesis I-Lisin oleh Brevibacterium Lactofermentum BL-1M76 di dalam Medium Molase

Abstract

!ho kinds of antibiotics, penicillin G and polymixin B sulphate, were used to affect the biosynthesis of L-Lysine by Brevibacterium BL-lM76 in molasses medium. Selection of appropriate antibiotic concentrations was carried out in two basal media (glucose and molasses media) in 250 ml Erknmeyer flasks containing 25 ml medium. The antibiotic concentmtwns examined mnged from 100-6000 unita/l. The most suituble antibiotic (jencillin G) and the most appropriate concentration (100 units/l) was applied to the scaled up process in 21 capacity fermentor (Eyela M-100 fermentor) which resulted in L-lysine production of 35.18 g/l.