Manipulasi kondisi fisiologis dan keasaman semen melalui pengaturan perbedaan kation anion ransum dalam upaya mempengaruhi karakteristik spermatozoa pada domba garut

Abstract

This study was carried out to evaluate the effect of dietary cation-anion difference (DCAD) and fish oil supplementation on dry matter intake, dry matter and organic matter digestibility, weight gain, acidity of urine, blood and semen, blood gas, plasma mineral, sperm characteristics and ratio of X:Y spermatozoa. The dietary treatmens were as follows: RN0= basal ration (DCAD +14) without fish oil, RNI= basal ration (DCAD +14) with 3% fish oil, RB0= base ration (DCAD +40) without fish oil, RBI= base ration (DCAD +40) with 3% fish oil, RA0= acid ration (DCAD -40) without fish oil, and RAI= acid ration (DCAD -40) with 3% fish oil. The rations contained 150 ppm of zinc and were offered to 18 of Garut breeed rams. The result indicated that: DCAD +40 and -40 decreased dry matter intake significantly. The ration with DCAD +40 had the lowest dry matter intake. No differences were observed for dry matter and organic matter digestibility. DCAD +40 and -40 decreased body weight of rams except RA0 ration, however, the body weight of rams was very low. Variation of urine pH followed the DCAD pattern. Blood pH, pCO2, and pO2 were not affected by DCAD, but cHCO3 and cBase were affected. DCAD affected plasma Mg and S, but did not affect K, Na, Zn and Cl. Semen pH after day 28 of experimental period were significantly different and that followed DCAD pattern. There were not differences for X:Y ratio, macroscopic and microscpic characteristic of sperm.