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      Eksplorasi dan Identifikasi Nematoda Entomopatogen pada Berbagai Tipe Tegakan di Hutan Pendidikan Gunung Walat.

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      Date
      2026
      Jenis/Type
      Skripsi
      Subtype
      Undergraduate Theses
      Author
      Usmawan, Dendi Rayendra Tri
      Rusniarsyah, Lufthi
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      Abstract
      Nematoda entomopatogen merupakan kelompok nematoda parasit serangga yang berperan sebagai agens hayati dan berpotensi dimanfaatkan sebagai insektisida biologi karena memiliki kemampuan untuk menginfeksi serta membunuh serangga inangnya dalam waktu relatif singkat. Keberadaan nematoda entomopatogen ini dipengaruhi oleh kondisi lingkungannya, baik faktor klimatis maupun edafis. Eksplorasi pada penelitian ini dilakukan menggunakan metode insect bait dengan larva Tenebrio molitor. Pemurnian dilakukan dengan metode White trap pada air steril, kemudian dilakukan penghitungan kepadatan populasi Juvenil Infektif (JI/mL). Identifikasi dilakukan berdasarkan karakter morfologinya. Penelitian ini memperoleh dua jenis nematoda entomopatogen yang teridentifikasi sebagai Steinernema sp. dan Heterorhabditis sp.. Nematoda dari tegakan agathis menunjukkan rata-rata kepadatan juvenil infektif terbanyak sebesar 3.030,5 JI/mL, tegakan pinus sebesar 1.531,5 JI/mL, dan tegakan puspa sebesar 1.768 JI/mL. Nematoda dari tegakan pinus menunjukkan rata-rata persentase infeksi tertinggi sebesar 38% dibandingkan tegakan agathis dan puspa. Keberadaan nematoda entomopatogen berkorelasi negatif kuat dengan suhu udara dan berkorelasi positif kuat dengan ketebalan serasah menandakan bahwa kondisi mikroklimat yang lebih lembap dapat mendukung sebagai habitat alami perkembangan nematoda entomopatogen.
       
      Entomopathogenic nematodes are a group of insect parasitic nematodes that act as biological agents and have the potential to be used as biological insecticides because of their ability to infect and kill their insect hosts in a relatively short period of time. The presence of these entomopathogenic nematodes is influenced by environmental conditions, including both climatic and edaphic factors. The exploration in this study was conducted using the insect bait method with Tenebrio molitor larvae. Purification was performed using the White trap method in sterile water, followed by a count of the population density of Infective Juveniles (IJ/mL). Identification was based on morphological characteristics. This study identified two species of entomopathogenic nematodes Steinernema sp. and Heterorhabditis sp.. Nematodes from the Agathis stand showed the highest average density of infective juveniles at 3.030,5 JI/mL, followed by the pine stand at 1.531,5 JI/mL, and the Puspa stand at 1.768 JI/mL. Nematodes from the pine stands showed the highest average infection rate of 38% compared to the Agathis and Puspa stands. The presence of entomopathogenic nematodes showed a strong negative correlation with air temperature and a strong positive correlation with litter depth, indicating that a more humid microclimate may serve as a natural habitat supporting the development of entomopathogenic nematodes.
       
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      http://repository.ipb.ac.id/handle/123456789/180151
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      Copyright © 2020 Library of IPB University
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      Contact Us | Send Feedback
      Indonesia DSpace Group 
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