Analisis Ekspresi Vimentin pada Hati Babi yang Diinduksi Sirosis oleh Diethylnitrosamine dan Phenobarbital.

Abstract

ARKHANA PRASETYO ABIMANYU. Analisis Ekspresi Vimentin pada Hati Babi yang Diinduksi Sirosis oleh Diethylnitrosamine dan Phenobarbital. Dibimbing oleh VETNIZAH JUNIANTITO dan SAVITRI NOVELINA. Sirosis hati merupakan kerusakan hati tahap lanjut yang ditandai fibrosis ekstensif dan nodul regeneratif. Model babi dipilih karena kemiripan anatomi dan fisiologi hatinya dengan manusia. Penelitian ini menganalisis ekspresi vimentinpenanda sel mesenkimal terkait aktivasi hepatic stellate cells (HSC) pada hati babi yang diinduksi sirosis menggunakan DENA dan phenobarbital, melalui pewarnaan HE, Azan-Mallory, dan Immunohistokimia. Hasil menunjukkan perubahan histopatologi berupa septa fibrotik, nodul regeneratif, dan deposisi kolagen di area septa, perivaskuler, dan perisinusoidal. Ekspresi vimentin dominan ditemukan di area tersebut dengan morfologi sel spindle-shaped, mengindikasikan aktivasi sel mesenkimal dan fibrogenesis aktif selama perkembangan sirosis. Temuan ini relevan sebagai acuan pengembangan diagnostik dan terapi penyakit hati kronis pada manusia.

ARKHANA PRASETYO ABIMANYU. Analysis of Vimentin Expression in Swine Liver Cirrhosis Induced by Diethylnitrosamine and Phenobarbital. Supervised by VETNIZAH JUNIANTITO and SAVITRI NOVELINA. Liver cirrhosis is an advanced stage of liver damage characterized by extensive fibrosis and regenerative nodules. The pig model was selected due to its anatomical and physiological similarities to the human liver. This study analyzed the expression of vimentin a mesenchymal cell marker associated with hepatic stellate cell (HSC) activationin pig livers induced with cirrhosis using DENA and phenobarbital, through HE staining, Azan-Mallory, and immunohistochemistry. Results showed histopathological changes including fibrous septa formation, regenerative nodules, and collagen deposition in septal, perivascular, and perisinusoidal areas. Vimentin expression was predominantly found in these areas with spindle-shaped cell morphology, indicating active mesenchymal cell activation and fibrogenesis during cirrhosis progression. These findings provide a relevant vimentin expression profile in a large animal model for the development of diagnostic and therapeutic approaches for chronic liver disease in humans.