Karakterisasi Citra Radiografi Kontras Implan Hormon Intravaginal Terbiodegradasi secara In Vitro dan In Vivo pada Domba Garut

Abstract

Peningkatan permintaan domba mendorong pengembangan teknologi reproduksi yang lebih efisien, salah satunya melalui penggunaan implan hormon intravaginal terbiodegradasi sebagai sistem sinkronisasi estrus tanpa proses pencabutan setelah penggunaan. Penelitian ini bertujuan menilai proses biodegradasi implan hormon intravaginal melalui karakterisasi citra radiografi dengan penambahan kontras iopamidol secara in vitro dan in vivo pada domba Garut. Pengujian in vitro dilakukan melalui perendaman implan pada media NaCl 0,9% selama 9 hari, sedangkan pengujian in vivo dilakukan dengan pengambilan citra radiografi bagian pelvis domba. Hasil in vitro menunjukkan terjadinya perubahan dimensi dan bobot, serta penurunan densitas radiografi implan seiring larutnya bahan kontras ke cairan supernatan, dengan formulasi implan multihormone (P4-P4-PGF2a) berlapis ganda kombinasi polimer polikaprolakton (PCL), polietilen glikol (PEG), dan kitosan memiliki stabilitas paling baik dibandingkan formulasi lainnya. Hasil in vivo menunjukkan bahwa implan mengalami penurunan visibilitas radiografi seiring waktu, dengan formulasi implan multihormone memiliki visibilitas radiografi yang paling jelas hingga hari ke-9. Penambahan bahan kontras iopamidol memungkinkan pemantauan biodegradasi implan hormon intravaginal secara radiografis, dengan formulasi implan multihormone (P4-P4-PGF2a) berlapis ganda PCL-PEG-kitosan menunjukkan stabilitas dan visibilitas radiografi paling baik selama pengujian in vitro dan in vivo.

The increasing demand for sheep has encouraged the development of more efficient reproductive technologies, one of which is the use of biodegradable intravaginal hormone implants as an estrus synchronization system that eliminates the need for implant removal after use. This study aimed to evaluate the biodegradation process of intravaginal hormone implants through radiographic image characterization using the addition of the contrast agent iopamidol under both in vitro and in vivo conditions in Garut sheep. The in vitro evaluation was conducted by immersing the implants in 0.9% NaCl solution for nine days, while the in vivo evaluation was performed by acquiring radiographic images of the pelvic region of the sheep. The in vitro results demonstrated changes in implant dimensions and weight, as well as a decrease in radiographic density over time due to the dissolution of the iopamidol contrast agent into the supernatant. The doublelayer-coated multihormone implant formulation (P4-P4-PGF2a) with polycaprolactone (PCL), polyethylene glycol (PEG), and chitosan exhibited the highest stability compared with the other formulations. The in vivo results showed a gradual reduction in implant radiographic visibility over time, with the multihormone implant formulation maintaining the clearest radiographic visibility up to day 9. The addition of the iopamidol contrast agent enabled radiographic monitoring of intravaginal hormone implant biodegradation, while the doublelayer-coated multihormone implant formulation (P4-P4-PGF2a) containing PCL, PEG, and chitosan demonstrated the greatest stability and radiographic visibility throughout both the in vitro and in vivo evaluations.