| dc.description.abstract | Cockroaches are important mechanical vectors that can spread microorganisms in
food-handling environments. Traditional meat markets often have poor sanitation,
increasing the risk of bacterial contamination. This study aimed to identify bacteria
present in cockroach faeces collected from meat stalls in Pasar Bogor using
molecular methods. Cockroaches were collected, and kept for obtaining faecal
samples, and bacterial DNA was analysed through partial 16S rRNA gene
sequencing. Sequence identification was performed using NCBI BLAST, and
phylogenetic analysis was conducted with the Neighbour-Joining method in MEGA
X. Four out of 16 sample pools were positive for bacteria, namely samples 13 through
16, with samples 15 and 16 being closely related to Psychrobacter spp, sample 13
being similar to an uncultured bacterium clone and sample 14 being related to
Halomonas sp.. Phylogenetic analysis of sample 15 and 16 confirmed their
clustering within the Psychrobacter clade with moderate to strong bootstrap
support. These findings suggest environmental contamination in meat stall settings
and highlight the potential role of cockroaches in bacterial dissemination,
emphasizing the need for improved hygiene and pest control measures. | |
