RESISTANSI ANTIBIOTIK ISOLAT Escherichia coli DARI DAGING AYAM BAKAR DI LINGKAR KAMPUS DRAMAGA IPB UNIVERSITY

Abstract

Ayam bakar termasuk pangan hewani yang rentan terkontaminasi bakteri Escherichia coli (E. coli) yang resistan terhadap antibiotik. Bakteri ini mengancam kesehatan publik karena sulit dikendalikan penyebarannya sebagai agen penyebab infeksi. Penelitian ini bertujuan untuk mengevaluasi tingkat resistansi antibiotik pada isolat E. coli dari daging ayam bakar yang dijual di sekitar lingkar Kampus Dramaga, IPB University. Uji resistansi dilakukan dengan metode Kirby-Bauer disk diffusion sesuai pedoman Clinical Laboratory Standards Institute (CLSI) 2023. Sebanyak 12 isolat yang teridentifikasi sebagai E. coli, tingkat resistansi tertinggi diperlihatkan terhadap eritromisin (50%), tetrasiklin (16%), sulfometoksazol-trimetoprim 8%), seftasidim (16%), streptomisin (8%), dan ampisilin (8%). Ditemukan isolat E. coli dengan sifat multidrug-resistant (MDR) sebesar 16% yang menunjukkan pola resistansi terhadap tiga dan empat kelas antibiotik. Tingginya resistansi antibiotik pada isolat E. coli dapat memicu masalah kesehatan masyarakat, karena bakteri resistan ini berpotensi menyebar melalui makanan terkontaminasi, sehingga meningkatkan risiko infeksi yang sulit diobati.

Grilled chicken is an animal-derived food susceptible to contamination by antibiotic-resistant Escherichia coli (E. coli). This bacterium poses a public health threat due to its difficult-to-control spread as an infectious agent. This study aimed to evaluate the level of antibiotic resistance in E. coli isolates from grilled chicken sold around the Dramaga Campus, IPB University. Resistance testing was conducted using the Kirby-Bauer disk diffusion method in accordance with the Clinical and Laboratory Standards Institute (CLSI) 2023 guidelines. Of the 12 isolates identified as E. coli, the highest resistance rates were recorded against erythromycin (50%), tetracycline (16%), sulfomethoxazole-trimethoprim (8%), ceftazidime (16%), streptomycin (8%), and ampicillin (8%). E. coli isolates were identified, with 16% exhibiting multidrug-resistant (MDR) characteristics and showing resistance patterns against three and four classes of antibiotics. The high level of antibiotic resistance in E. coli isolates may trigger public health issues, as these resistant bacteria have the potential to spread through contaminated food, thereby increasing the risk of difficult-to-treat infections