Aplikasi Hormon 17a-Metiltestosteron (MT) dalam Proses Maskulinisasi Ikan Killifish Guentheri Red Nothobranchius guentheri

Abstract

Ikan killifish guentheri red (Nothobranchius guentheri) merupakan ikan hias air tawar bernilai ekonomis tinggi, karena warna tubuh ikan jantan lebih menarik dibandingkan betina. Tujuan penelitian ini untuk mengevaluasi penggunaan hormon 17a-metiltestosteron terhadap persentase ikan jantan, sintasan, dan tingkat abnormalitas. Penelitian menggunakan hormon 17a-metiltestosteron (MT) dengan rancangan acak lengkap dengan 4 perlakuan dan 3 ulangan, yaitu H0 (tanpa hormon), H1 (5 mg L-1), H2 (10 mg L-1), dan H3 (15 mg L-1) melalui perendaman larva selama 6 jam. Hasil menunjukkan bahwa pemberian hormon MT berpengaruh signifikan (P<0,05) terhadap persentase ikan jantan dan sintasan, namun tidak berpengaruh signifikan (P>0,05) terhadap tingkat abnormalitas. Persentase ikan jantan tertinggi diperoleh pada perlakuan H2 sebesar 91,83±8,61% dan sintasan tertinggi pada perlakuan H0 sebesar 76,33±2,36%. Tingkat abnormalitas ikan hanya ditemukan pada perlakuan H3 sebesar 0,33±0,29%. Dosis hormon 10 mg L-1 merupakan dosis paling efektif dalam meningkatkan persentase ikan jantan.

Killifish guentheri red (Nothobranchius guentheri) is a freshwater ornamental fish with high economic value due to the more attractive coloration of males compared to females. This study aimed to evaluate the application of 17a-methyltestosterone (MT) on male percentage, survival rate, and abnormality rate. The experiment was conducted using a completely randomized design with four treatments and three replications, namely H0 (without hormone), H1 (5 mg L-1), H2 (10 mg L-1), and H3 (15 mg L-1) applied through larval immersion for 6 hours. The results showed that MT administration had a significant effect (P<0,05) on male percentage and survival rate, but no significant effect (P>0,05) on abnormality percentage. The highest percentage of male fish was obtained in treatment H2 at 91,83±8,61% and the highest survival rate was observed in H0 at 76,33±2,36%. Abnormalities were only found in H3 at 0,33±0,29%. The dose of 10 mg L-1 was the most effective in increasing the percentage of male fish.