Deteksi Cemaran Bakteri pada Lingkungan Kerja di Laboratorium Kesmavet DKI Jakarta

Abstract

SESSY NABILAH RAMADHAYANI. Deteksi Cemaran Bakteri pada Lingkungan Kerja di Laboratorium Kesmavet DKI Jakarta. Dibimbing oleh ERNI SULISTIAWATI Laboratorium mikrobiologi memiliki fungsi yang sangat penting dalam pengujian sampel biologis, termasuk dalam mendeteksi mikroorganisme patogen pada hewan dan produk hewani. Risiko kontaminasi mikroba di lingkungan kerja dapat memengaruhi akurasi pengujian. Penelitian ini bertujuan untuk mendapatkan informasi tingkat cemaran pada lingkungan kerja di Laboratorium Mikrobiologi Kesmavet DKI Jakarta. Penelitian berlangsung selama 5 hari dengan 10 sampel cemaran mikroba, terdiri dari 5 sampel udara (SWR 1-5) dan 5 sampel usap ruang (SW 1-5). SW diuji triplo dengan metode pour plate, sedangkan SWR diuji simplo. Perhitungan pertumbuhan koloni pada pengujian Total Plate Count (TPC) dihitung menggunakan Colony Forming Unit (CFU). Hasil menunjukkan jumlah koloni udara lebih tinggi dibandingkan permukaan ruang (p = 0,022), mengindikasikan udara sebagai sumber kontaminasi dominan. Faktor seperti aliran udara, kebersihan lingkungan, dan sumber kontaminasi eksternal berpengaruh terhadap cemaran. Peningkatan sanitasi, pemantauan kualitas udara, dan teknologi deteksi real-time direkomendasikan untuk menjaga kebersihan laboratorium dan meningkatkan keandalan pengujian

SESSY NABILAH RAMADHAYANI. Detection of Bacterial Contamination in the Kesmavet Laboratory Work Environment, DKI Jakarta. Supervised by ERNI SULISTIAWATI Microbiology laboratories play a crucial role in the analysis of biological samples, includes the identification of potentially harmful microorganisms in animals and animal products. The risk of microbial contamination in the workplace can affect testing accuracy. This study aims to assess the level of contamination in the working environment of the Kesmavet Microbiology Laboratory in DKI Jakarta. The research was conducted 5 days using 10 microbial contamination samples, consisting of 5 air samples (SWR 1-5) and 5 surface swab samples (SW 1-5). SW samples were tested in triplicate using the pour plate method, while SWR samples were tested in a single replicate. The Total Plate Count (TPC) was determined using the Colony Forming Unit (CFU) method. The results showed that airborne colony counts were significantly higher than those on surface swabs (p = 0.022), indicating that air is the dominant source of contamination. Factors such as airflow, environmental cleanliness, and external contamination sources influence contamination levels. Enhancing sanitation procedures, monitoring air quality, and implementing real-time detection technology are recommended to maintain laboratory hygiene and improve testing reliability.