dc.contributor.advisor | Indriastuti, Cecilia Eny | |
dc.contributor.advisor | Sulistiani,, Erina | |
dc.contributor.author | YULISTIANA, ARTYA VIONA TRY | |
dc.date.accessioned | 2024-08-14T08:28:38Z | |
dc.date.available | 2024-08-14T08:28:38Z | |
dc.date.issued | 2024 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/157374 | |
dc.description.abstract | Permintaan planlet rumput laut kotoni yang tinggi perlu dipenuhi dalam
waktu yang efisien merupakan tantangan dalam kultur jaringan rumput laut.
Penelitian ini bertujuan untuk mencari metode kultur yang optimal serta mengamati
pengaruh penambahan sitokinin BAP pada rumput laut kotoni Kappaphycus
alvarezii. Metode yang digunakan yaitu menggunakan Rancangan acak lengkap
(RAL) dengan 4 perlakuan dan 10 kali ulangan. Media yang digunakan yaitu media
cair dengan penambahan pupuk PES (20 ml/L) serta ZPT BAP yang terbagi menjadi
B1 (0,1 mg/L), B2 (0,2 mg/L), B3 (0,3 mg/L) serta Kontrol (tanpa BAP). Hasil
penelitian menunjukan perlakuan BAP 0,1 mg/L memiliki nilai kemunculan
mikropropagul tertinggi 1,8 individu, persentase tumbuh 100% dan jumlah rata-rata
54 individu. Simpulan dari penelitian ini menunjukan pemberian ZPT BAP dengan
konsentrasi 0,1 mg/L pada media cair menghasilkan respon yang terbaik terhadap
taraf kecepatan pertumbuhan kalus menjadi mikropropagul dan menghasilkan
jumlah mikropropagul lebih banyak. Sehingga dinilai efektif untuk perangsangan
induksi regenerasi kalus menjadi mikropropagul. | |
dc.description.abstract | Request plantlets grass sea high cotony need fulfilled in efficient time is
challenge in tissue culture grass sea . This research aims to find optimal culture
methods and observe the effect of adding the cytokinin BAP to the kotoni seaweed
Kappaphycus alvarezii . The method used was a completely randomized design
(CRD) with 4 treatments and 10 replications. The media used is liquid media with
the addition of PES fertilizer (20 ml/L) and ZPT BAP which is divided into B1 (0.1
mg/L), B2 (0.2 mg/L), B3 (0.3 mg/L ) as well as Control (without BAP). The
research results showed that the 0.1 mg/L BAP treatment had the highest
micropropagule emergence value of 1.8 individuals, a growth percentage of 100%
and an average number of 54 individuals. The conclusions from this research show
that administering ZPT BAP with a concentration of 0.1 mg/L in liquid media
produces the best response to the rate of callus growth rate into micropropagules
and produces a greater number of micropropagules. So it is considered effective for
stimulating the induction of callus regeneration into micropropagules. | |
dc.description.sponsorship | | |
dc.language.iso | id | |
dc.publisher | IPB University | id |
dc.title | Optimalisasi Pertumbuhan Rumput Laut Kotoni Kappaphycus alvarezii dengan Penambahan Zat Pengatur Tumbuh (ZPT) Sitokinin Benzylaminopurin (BAP) | id |
dc.title.alternative | Optimization Growth Kotoni Seaweed Kappaphycus alvarezii with Addition Substance Regulator Growth (ZPT) Cytokinin Benzylaminopurine (BAP) | |
dc.type | Tugas Akhir | |
dc.subject.keyword | micropropagule | id |
dc.subject.keyword | BAP | id |
dc.subject.keyword | callus | id |
dc.subject.keyword | benzylaminopurine | id |