Kualitas Semen Beku Sapi Friesian Holstein Menggunakan Pengencer Tris Kuning Telur dan Penambahan Autologous Platelet-Rich Plasma

Abstract

Pengencer Tris Kuning Telur (TKT) dan penambahan aPRP secara keseluruhan mampu mempertahankan kualitas semen selama proses kriopreservasi. Penelitian bertujuan membandingkan kualitas semen beku sapi FH menggunakan pengencer Tris Kuning Telur (TKT) dan penambahan Autologous Platelet-Rich Plasma. Autologous Platelet-Rich Plasma dibuat dengan metode sentrifugasi selama 20 menit. Semen dikoleksi menggunakan vagina buatan, dikumpulkan dan dibagikan kedalam 5 alikuot, diencerkan dengan pengencer kuning telur-gliserol tris yang ditambahkan dengan proporsi aPRP yang berbeda [0% (kontrol), 2%, 5%, 10%, dan 15%] dilanjutkan dengan kriopreservasi menggunakan prosedur standar. Semen diekuilibrasi selama 3 jam, kemudian dibekukan dengan menggunakan uap nitrogen (N2) cair dan disimpan selama satu hari dalam kontainer N2 cair. Semen beku selanjutnya dithawing selama 30 detik di dalam air suhu 37 oC dan dievaluasi kualitasnya terhadap karakteristik motilitas spermatozoa dengan metode estimasi dan CASA, viabilitas spermatozoa, membran plasma utuh (MPU), dan recovery rate (RR). Hasil penelitian menunjukkan bahwa perbedaan nyata (P<0,05) ditemukan pada motilitas spermatozoa, viabilitas spermatozoa, MPU, dan RR. Inseminasi buatan diperlukan untuk meningkatkan kualitas dan populasi sapi perah Friesian Holstein di Indonesia. Tris kuning telur dengan aPRP 5% merupakan pengencer dengan konsentrasi terbaik pada semen sapi FH selama proses kriopreservasi.

Tris egg yolk with aPRP diluents were overall able to maintain semen quality during the cryopreservation process. The study aimed to compare the quality of frozen semen of FH cattle using Tris Egg Yolk (TKT) diluent with Autologous Platelet-Rich Plasma. Autologous Platelet-Rich Plasma was made by centrifugation method for 20 minutes. Semen was collected using artificial vagina, pooled and divided into 5 aliquots, diluted with Tris egg yolk-glycerol diluent supplemented with different proportions of aPRP [0% (control), 2%, 5%, 10%, and 15%] followed by cryopreservation using standard procedures. Semen was equilibrated for 3 hours, then frozen using liquid nitrogen (N2) vapor and stored for one day in a liquid N2 container. Frozen semen was then thawed for 30 seconds in 37 oC water and its quality was evaluated against the characteristics of spermatozoa motility by the estimation method and CASA, spermatozoa viability, intact plasma membrane (MPU), and recovery rate (RR). The results showed that significant differences (P <0.05) were found in spermatozoa motility, spermatozoa viability, MPU, and RR. Artificial insemination is needed to improve the quality and population of Friesian Holstein dairy cattle in Indonesia. Tris egg yolk with 5% aPRP was the diluent with the best concentration in FH cow semen during the cryopreservation process.