dc.contributor.advisor | Setiyono, Agus | |
dc.contributor.advisor | Juniantito, Vetnizah | |
dc.contributor.author | Purnamiharja, Tsulatsy Puspitasari | |
dc.date.accessioned | 2024-02-28T00:29:58Z | |
dc.date.available | 2024-02-28T00:29:58Z | |
dc.date.issued | 2024 | |
dc.identifier.uri | http://repository.ipb.ac.id/handle/123456789/140219 | |
dc.description.abstract | Query fever (Q fever) merupakan penyakit zoonosis yang disebabkan oleh bakteri Gram-negatif bersifat obligat intraseluler yaitu Coxiella burnetii. Hewan ternak menjadi sumber utama penularan pada hewan lainnya maupun manusia. Sapi termasuk salah satu hewan sangat rentan terhadap infeksi akan tetapi umumnya tidak menunjukkan gejala klinis. C. burnetii pada sapi dapat menyebabkan abortus dan beberapa gangguan reproduksi lainnya. Distribusi C. burnetii sudah menyebar luas hampir ke seluruh dunia termasuk di Indonesia, dan penularan utamanya melalui aerosol. Studi dan laporan mengenai kasus Q fever pada hewan di Indonesia masih sangat terbatas hingga saat ini karena diagnosa klinis yang sulit dilakukan untuk deteksi dini. Kabupaten Bandung adalah salah satu wilayah di Jawa barat yang rentan terhadap penyebaran Q fever karena jumlah ternak yang tinggi khususnya sapi. Penelitian ini bertujuan untuk mendeteksi keberadaan C. burnetii sebagai penyebab Q fever pada berbagai organ sapi di Kabupaten Bandung.
Sampel organ diambil dari 240 ekor sapi yang dipotong di empat Rumah Potong Hewan (RPH) di Kabupaten Bandung. Metode yang digunakan untuk mendeteksi keberadaan C. burnetii dalam sampel adalah nested Polymerase Chain Reaction (PCR) menggunakan primer outer membrane protein (OMP). Sampel positif hasil nested-PCR dilakukan pembuatan preparat histopatologi dengan pewarnaan hematoksilin-eosin (HE) untuk analisis lesi patologi. Lima ekor (2,08%) sapi Brahman cross terdeteksi positif C. burnetii yang berada dalam tiga organ jantung (1,25%) dan dua organ paru (0,83%). Gambaran histopatologi secara umum tidak menunjukkan adanya lesi signifikan dan spesifik terhadap infeksi C. burnetii melalui pewarnaan HE. Berdasarkan hasil tersebut, penelitian ini mengonfirmasi adanya infeksi subklinis C. burnetii pada populasi sapi di Kabupaten Bandung tanpa disertai lesi spesifik. Penelitian lanjutan mengenai Q fever diharapkan dapat dilakukan dengan beberapa metode lain seperti pewarnaan imunohistokimia atau sequencing. | id |
dc.description.abstract | Coxiella burnetii, an obligate intracellular Gram-negative bacterium, is responsible for causing the zoonotic disease called Q fever. Livestock animals serve as the primary reservoir of infection for both animals and humans. Cattle are highly prone to infection; however, they typically remain asymptomatic. Coxiella burnetii in cattle can result in abortion and various other reproductive problems. C. burnetii has achieved global distribution, encompassing Indonesia, and its primary mode of transmission is by aerosols. There is a scarcity of studies and reporting on instances of Q fever in animals in Indonesia due to the challenging nature of clinical diagnosis for early detection. Bandung Regency, located in West Java, is susceptible to the transmission of Q fever due to its significant animal population, particularly cattle. The objective of this study is to identify the existence of C. burnetii as the etiological agent responsible for Q fever in cattle in Bandung Regency.
Total 240 cattle slaughtered at four slaughterhouses (RPH) in Bandung Regency were used to collect organ samples. The technique employed for identifying the existence of C. burnetii in samples is nested polymerase chain reaction (PCR) with primers specific to the outer membrane protein (OMP). The PCR-positive samples were processed into histopathology slides and stained with hematoxylin-eosin (HE) for the examination of pathological abnormalities. Out of the total of five Brahman cross cattle, which accounts for 2,08% of the sample, three hearts (1,25%) and two lungs (0,83%) were found to be positive for C. burnetii. The histological investigation did not reveal any notable or distinctive lesions indicative of C. burnetii infection when using HE staining. This investigation has verified the occurrence of subclinical C. burnetii infection in the cattle population in Bandung Regency, albeit in the absence of particular symptoms. Additional investigations on Q fever can be conducted utilizing alternative techniques, such as immunohistochemical labeling or sequencing. | id |
dc.language.iso | id | id |
dc.publisher | IPB University | id |
dc.title | Deteksi Molekuler Coxiella burnetii Penyebab Zoonosis Q Fever pada Sapi di Rumah Potong Hewan Kabupaten Bandung | id |
dc.title.alternative | Molecular Detection of Coxiella burnetii Causes of Zoonotic Q Fever in Cattle at the Bandung Regency Slaughterhouse | id |
dc.type | Thesis | id |
dc.subject.keyword | Q fever | id |
dc.subject.keyword | Coxiella burnetii | id |
dc.subject.keyword | nested-PCR | id |
dc.subject.keyword | cattle | id |