Uji Lapang Lacak Balak Kayu Jati dengan Penanda RAPD
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INTRODUCTION. Demand for teak based product increases progressively, particularly as raw materials for furniture industry. The increasing demand for teak wood, exceeds the amount that can be produced sustainably by production forest. The gaps existing between demand for raw material teak wood and the volume of teak wood which can be produced have triggered the activity of illegal logging and administrative manipulation of wood. Therefore there is a need for accurate method of producing evidence which is difficult to be manipulated to support log tracking and determine the origin of doubtful wood. The use of genetics technology is a new method to be developed and applied for log tracking certification and evidence seeking for criminal cases, such as illegal logging. Genetic markers are inherent and internal features of the wood itself, so they are difficult to be manipulated. One of the methods of genetic marking which could be applied for DNA analysis was RAPD (Random Amplified Polymorphic DNA). The objectives of this research were testing the matching between stump wood DNA and wood DNA in log landing site (TPK), and approximate detection of the origins of stolen woods and woods which are used for sawmill industry, based on existing database. MATERIALS AND METHOD. This research was conducted in Room of Genetic Analysis, Sub-department of Silviculture, Faculty of Forestry; and Laboratory of Molecular Biology, Inter University Center (PAU), Bogor Agricultural University, from August through October 2008. Plant materials used were woods from KPH (Forest Management Unit) Purwakarta (stump wood and TPK wood) and KPH Ciamis (stump wood, TPK wood, stolen wood and sawmill industry wood). The method used was RAPD (Random Amplified Polymorphic DNA). Equipments used were among other things, tube, micropipette, tips, centrifugation, freezer, electrophoresis bath, PCR machine and UV transilluminator. Materials used were buffer extract, PVP, chloroform, phenol, ethanol, aquabidest, DNA, primer, H2O, taq polymerase, agarose, buffer TAE and blue juice. Tracking of stump wood and TPK wood was conducted on the basis of allelic structure of individual stump woods and TPK woods which were tested. Approximate detection of the origin of stolen wood and sawmill wood was conducted by cluster analysis. RESULTS AND CONCLUSION. Results of log tracking showed similarity in allelic structure between stump wood and TPK wood in teak populations of Ciamis but not for population of Purwakarta. This phenomenon showed DNA technology can be used to match wood DNA between stump and that in TPK. Results of cluster analysis showed that stolen woods and woods used in sawmill industry grouped themselves in West Java cluster, or specifically in cluster KPH Ciamis with least genetic distance of 0.0353 for stolen woods and 0.0358 for woods used in sawmill industry.
- UT - Silviculture